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Dr. Jan Teisinger, CSc.


ResearcherID: B-7122-2012

 

Function

Senior researcher

 

Areas of research

Structural and functional detarminants on TRPV channels.  Transient receptor potential (TRP) channel family consists of major families TRPV, TRPC, TRPM and TRPA1, which share a common topology. The cytoplasmic N- and C-tails are separated by six predicted transmembrane domains (TM1-6) including a pore between TM5-6. They are regulated by intracellular ligands like calmodulin and phosphatidyl inositol-4,5-bi-phosphate (PIP2). The aims are: to determine binding sites for calmodulin in both intracellular N- and C-tails of channels TRPV2,3,5, in N-tail of TRPV4 and binding sites for PIP2 in TRPV2,3,4,6 in both N- and C-tails and in N-terminus TRPV1 and 5. Fusion proteins of both N- and C-tails will be obtained for binding studies using fluorescence spectroscopy. Another aim will be describing the role in gating process of given amino acids residues localized in the pore between TM5-6 in TRPV2,3,4 and to clarify the role of the of aminoacids residues in the lower part of TM6. To confirm electrophysiological data on TRPV2,3,4 and their mutants, the homology model of TRPV channels will be created on base KcsA bacterial channel template.

 

Education

1991 CSc.           Biochemistry, Charles University, 1st Faculty of Medicine, Prague

1968 Ing.             Chemistry, Institute of Chemical Technology, Prague

 

E-mail: teisingr@fgu.cas.cz, tel: 00420241062740