Intranet Čeština

Adipose Tissue Biology

Adipose Tissue Biology

Our department is studying physiological regulations of metabolisms and their disturbances in obesity and associated diseases (i.e. Metabolic syndrome). In order to help treat these disorders we explore the influence of drugs, diet and natural substances, namely n-3 polyunsaturated fatty acids of marine origin (omega-3). Our results show the importance of the metabolism of adipose tissue for accumulation of body fat and reveal new possibilities in the prevention and treatment of metabolic disorders linked to obesity by modulating adipose tissue metabolism. We investigate mechanisms that regulate metabolism in health and disease by combining experiments on mice and cell models with clinical studies, and we try to apply new knowledge in clinical medicine. While working with the manufacturers of the tested substances in the Czech Republic and Norway, we investigate the possible use of omega-3 to increase the effect of drugs and other substances in the treatment of selected diseases. Research projects of the department reflect the current grant support and the existence of Research units established during 2015. The "Energy metabolism unit" (PI: J. Kopecký) examines the importance of a "futile" cycle involving the triglyceride hydrolysis and re-esterification of fatty acids in adipocytes of white adipose tissue for resistance to obesity and associated metabolic disorders, namely in the context of calorie restriction, omega-3 administration and cold exposure. The influence of the above manipulations on the formation of lipid mediators, mitochondrial metabolism and proliferation of cells in the adipose tissue is also being characterized. The "Metabolomics unit" (PI: O. Kuda) focuses on the analysis of lipid mediators (eicosanoids) in adipose tissue using the UPLC-MS / MS method, in particular to identify the source of production of various mediators and their effect on metabolism and inflammatory properties of adipose tissue cells (i.e. adipocytes vs. macrophages). New metabolomic methods are also being developed. The "Glucose homeostasis unit" (PI: M. Rossmeisl), by using the method of hyperinsulinemic-euglycemic clamps, analyzes the insulin-sensitizing effects of omega-3 depending on the lipid form (i.e. triacylglycerols vs. phospholipids) of their administration, as well as the involvement of the endocannabinoid system in these effects. We examine the changes in the intestine and their involvement in differential effects of various lipid forms of omega-3 on the whole-body metabolism. Translational potential regarding the beneficial effects of omega-3 on metabolism will also be tested using the transgenic mice expressing the human version of the transcription factor PPARalpha. All the above mentioned Research units are closely collaborating and are engaged in translational research conducted in collaboration with clinical as well as industrial partners.



The Department is subdivided into three Research units with complementary focus, which closely collaborate and are engaged in translational research conducted together with clinical as well as industrial partners.



Anti-inflammatory effects of novel lipokines of fatty acid esters of hydroxy fatty acids family in obesity

White adipose tissue (WAT) is a complex  endocrine organ  and its low-grade  inflammation in obesity  contributes to the development of metabolic  disorders. Omega-3 polyunsaturated fatty acids  (PUFA) play an important  role in resolution  of inflammation and exert beneficial  metabolic effects.  In 2014,  a class of WAT-born lipid mediators - fatty acid esters of hydroxy fatty acids  ( FAHFA) derived  from palmitic and stearic acid with anti-inflammatory  and anti-diabetic properties was discovered [link]. Our hypothesis is that novel FAHFAs derived  from omega-3 PUFA, with anti-inflammatory  properties, could be found in mice and humans and that they can beneficially affect adipose tissue metabolism in obesity,  especially low-grade  inflammation. Using experiments in cell cultures, mice and humans we will explore  the structures, effects  on WAT inflammation,  WAT glucose tolerance and molecular  mechanisms of signaling  of these new lipokines. Our results will presents a significant advance in research of the mechanisms connecting inflammation,  metabolism, and nutritional lipids. More

The role of intestine in differential metabolic effects of various lipid forms of dietary omega-3 fatty acids

Long-chain fatty acids of n-3 series (omega-3), mainly EPA and DHA of marine origin, may protect against obesity and exert hypolipidemic effects. While the mechanisms of omega-3’s effects on lipid metabolism in the liver are relatively well described, changes in metabolism in the intestine due to omega-3 are not entirely understood. Previous studies showed that metabolic effects are stronger when omega-3 are administered as phospholipids (Rossmeisl et al., PlosOne 2012). The main goal of this project is to identify the nature of differential metabolic effects of omega-3 depending on the lipid form of their administration (triacylglycerols, phospholipids or wax esters), mainly with regard to the involvement of the intestine in these effects.   More

Physiological relevance of white adipose tissue plasticity and its relationship to the development of obesity

Sufficient plasticity of white adipose tissue allows to adjust appropriately various metabolic processes in response to changing environmental conditions. Metabolic plasticity of adipose tissue is linked with changes in the size and content of cells within the tissue. A hypothesis will be verified that adipose tissue plasticity, namely the proliferative potential of adipose tissue cells, represents an important factor counteracting toxic effects of fatty acids that are released during the breakdown of lipid stores. Reduced ability of the organism to activate these processes could contribute to the  development of obesity and its metabolic consequences. More

Effects of omega-3 in type 2 diabetic patients – new mechanisms for targeted therapy

Minor dietary components with proven biological effects play an important role in the complex treatment strategy of obesity-related diseases, however our knowledge regarding the mechanisms of their action is relatively limited. The aim of this project is to identify novel mechanisms behind the beneficial effects of omega-3 in patients with type 2 diabetes mellitus (DM), i.e. the only dietary component with clearly demonstrated beneficial effects on dyslipidemia and cardiovascular mortality and morbidity in the diabetic patients.  More

The role of endocannabinoid system in metabolic effects of marine omega-3 phospholipids

Metabolic disturbances in obesity are associated with dysregulation of the endocannabinoid (EC) system. We have shown in mice fed an obesogenic high-fat diet that metabolic effects of long-chain omega-3 fatty acids were stronger when supplied in the phospholipid form (omega-3 PL) as compared with triacylglycerols (Rossmeisl et al. 2012. PloS One). These effects were associated with a more efficient modulation of major EC molecules in white adipose tissue (WAT). More



Kuda, Ondřej . Bioactive metabolites of docosahexaenoic acid . Biochimie. 2017, roč. 136, May, p. 12-20 . IF = 3.112 [ASEP] [ doi ]
Flachs, Pavel - Adamcová, Kateřina - Zouhar, Petr - Marques, C. - Janovská, Petra - Viegas, I. - Jones, J. G. - Bardová, Kristina - Svobodová, Michaela - Hansíková, Jana - Kuda, Ondřej - Rossmeisl, Martin - Liisberg, U. - Borkowska, A. G. - Kristiansen, K. - Madsen, L. - Kopecký, Jan . Induction of lipogenesis in white fat during cold exposure in mice: link to lean phenotype . International Journal of Obesity. 2017, roč. 41, 3, p. 372-380 . IF = 5.487 [ASEP] [ doi ]
Horáková, Olga - Hansíková, Jana - Bardová, Kristina - Gardlo, Alžběta - Rombaldová, Martina - Kuda, Ondřej - Rossmeisl, Martin - Kopecký, Jan . \\nPlasma Acylcarnitines and Amino Acid Levels As an Early Complex Biomarker of Propensity to High-Fat Diet-Induced Obesity in Mice . PLoS ONE. 2016, roč. 11, 5, e0155776 . IF = 2.806 [ASEP] [ doi ]
Slámová, Kristýna - Papoušek, František - Janovská, Petra - Kopecký, Jan - Kolář, František . Adverse Effects of AMP-Activated Protein Kinase alpha 2-Subunit Deletion and High-Fat Diet on Heart Function and Ischemic Tolerance in Aged Female Mice . Physiological Research. 2016, roč. 65, 1, p. 33-42 . IF = 1.461 [ASEP]
Rohm, M. - Schäfer, M. - Laurent, V. - Üstünel, B. E. - Niopek, K. - Algire, C. - Hautzinger, O. - Sijmonsma, T. P. - Zota, A. - Medrikova, D. - Pellegata, N. S. - Ryden, M. - Kulyte, A. - Dahlman, I. - Arner, P. - Petrovic, N. - Cannon, B. - Amri, E. Z. - Kemp, B. E. - Steinberg, G. R. - Janovská, Petra - Kopecký, Jan - Wolfrun, Ch. - Blüher, M. - Diaz, M. B. - Herzig, S. An AMP-activated protein kinase–stabilizing peptide ameliorates adipose tissue wasting in cancer cachexia in mice . Nature Medicine. 2016, roč. 22, 10, p. 1120-1130 . IF = 29.886 [ASEP] [ doi ]

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Institute of Physiology AS CR, v.v.i.
Department of Adipose Tissue Biology

Vídeňská 1083
14220 Prague 4
Czech Republic
tel. +420 241 062 554
fax. +420 241 062 599


MUDr. Martin Rossmeisl, Ph.D.
head of the department

MUDr. Jan Kopecký, DrSc.
deputy head of the department
Mgr. Olga Horáková, Ph.D.
senior researcher
Ing. Petra Janovská, Ph.D.
senior researcher
RNDr. Ondřej Kuda, Ph.D.
senior researcher
Mgr. Kristina Bardová, Ph.D.
junior researcher 
Mgr. Michaela Svobodová, Ph.D.
junior researcher 
Mgr. Kateřina Adamcová
PhD student
Mgr. Jana Hansíková
PhD student
Mgr. Petra Kuchaříková
PhD student
Mgr. Marie Březinová
PhD student
Mgr. Jana Pavlišová
PhD student (maternity leave)
Mgr. Martina Rombaldová
PhD student (maternity leave)

Jaroslava Bémová

Ilona Berková

Mgr. Ivana Brabcová, Ph.D.

Jana Jahelková
Ing. Adéla Krejcárková
Soňa Hornová

Daniela Šálková