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Analysis of Biologically Important Compounds

Analysis of Biologically Important Compounds Proteins of tooth pulp separated by 2D electrophoresis (excision) and stained with silver.

Main topics:

  • developing of new separation methods (capillary electromigration techniques), for example:
    • affinity electromigration methods
    • application of gold nanoparticles in capillary electromigration methods
    • forensic application (including coupling CE-MS)
  • analysis of connective tissues - proteomic and physiological analysis of biological samples   when many new proteins were identified, for example:
    • proteom of human teeth in connection to decoy
    • insoluble proteins from the avian eggshell, especially cuticle
    • analysis of posttraslational modifications of proteins
    • determination of arising compounds in glycation reaction as well as sites of modifications
    • identification of age-related changes in proteins – oxidation and deamidation of proteins
  • analysis of a broad spectrum of biologically important compounds in cooperation with many departments of our Institute, various Czech Institutes as well as international cooperation (e.g. proteins, steroids, pigments)
  • proteomic facility of Institute of Physiology CAS





Mikšík, Ivan - Pataridis, Statis - Eckhardt, Adam - Sedmera, David . Analysis of Siamese Crocodile (Crocodylus siamensis) Eggshell Proteome . Protein Journal 2018, roč. 37, 1, p. 21-37 . IF = 1.133 [ASEP]
Kohutiar, Matej - Eckhardt, Adam - Mikšík, Ivan - Šantorová, J. - Wilhelm, Jiří . Proteomic analysis of peroxynitrite-induced protein nitration in isolated beef heart mitochondria . Physiological Research 2018, roč. 67, 2, p. 239-250 . IF = 1.324 [ASEP]
Peševski, Živorad - Kvasilová, A. - Stopková, T. - Naňka, O. - Drobná Krejčí, Eliška - Buffinton, Ch. - Kočková, Radka - Eckhardt, Adam - Sedmera, David . Endocardial Fibroelastosis is Secondary to Hemodynamic Alterations in the Chick Embryonic Model of Hypoplastic Left Heart Syndrome . Developmental Dynamics 2018, roč. 247, 3, p. 509-520 . IF = 2.507 [ASEP] [ doi ]
Marschner, Karel - Musil, Stanislav - Mikšík, Ivan - Dědina, Jiří . Investigation of hydride generation from arsenosugars - Is it feasible for speciation analysis? . Analytica Chimica Acta 2018, roč. 1008, MAY, p. 8-17 . IF = 5.123 [ASEP] [ doi ]
Svobodová, Jana - Kofroňová, Olga - Benada, Oldřich - Král, V. - Mikšík, Ivan . Separation of oligopeptides, nucleobases, nucleosides and nucleotides using capillary electrophoresis/electrochromatography with sol–gel modified inner capillary wall . Journal of Chromatography A. 2017, roč. 1517, p. 185-194 . IF = 3.716 [ASEP] [ doi ]

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Institute of Physiology AS CR, v.v.i.
Department of Analysis of Biologically Important Compounds

Vídeňská 1083
14220 Prague 4
tel. +420 241 062 534
fax. +420 241 062 558





​​doc. Ing. Tomáš Čajka, Ph.D.

head of the department
  Mgr. Adam Eckhardt, Ph.D.
deputy head of the department
  Prof. Ing. Ivan Mikšík, DrSc.
senior researcher
  RNDr. Michal Jágr, Ph.D.
senior researcher
  Ing. Jana Svobodová, Ph.D.
senior researcher
  Mgr. Pavla Sedláková, Ph.D.
senior researcher
  Ing. Statis Pataridis
research assistant
  Mgr. Lucie Kulhavá
PhD student
  Mgr. Armenuhi Kirakosjanová
lab technician