Intranet Čeština



The Department of Biomathematics consists of two research groups: (1) Bioimaging and Image Analysis, and (2) Biochemistry of Membrane Receptors. Operating mainly at in situ and in vitro levels, respectively, the two groups complement each other when studying phenomena confined to the cell membrane.


1.      Bioimaging and Image Analysis

The group conducts research into the 3D microanatomical aspects of physiological phenomena at mesoscopic, microscopic and ultrastructural level. It is engaged in a range of collaborative projects across the campus and beyond. If interested please contact us (



  • Reconstruction and registration of images acquired by multimodal preclinical imaging methods
  • The conduction system of developing murine heart



  • Development of 3D image analysis and stereological methods including software (employed, e.g., in a volumetric analysis of Langerhans islets from human donors)
  • Microscopic optical-contrasting (label-free) strategies to visualize living cells and tissues


OTHER PROJECTS (selection only)

  • Effects of ionizing radiation on blood capillaries network
  • Effects of elevated CO2 on the photosynthetic apparatus’ performance and  ultrastructure
  • Mechanisms governing the formation of extracellular matrix proteins (collagen and elastin)



  • Laser scanning confocal microscopy
  • Two-photon excitation microscopy
  • Second harmonic generation (SHG) microscopy
  •  ‘White-light‘ laser and hybrid detectors (on Leica TCS SP8 WLL MP microscope)
  • FRAP recording, FRET analysis, FLIM/PLIM recording including λ2 scans 
  • Optical projection tomography (OPT)
  • Selective plane illumination (light sheet) microscopy (SPIM)
  • Phase-, modulation- and interference contrast microscopy



  • Software for 3D image analysis, stereology, spatial statistics and deconvolution (Hyughens)
  • Optical/chemical clearing protocols for OPT and SPIM



The group is part of the Czech BioImaging infrastructure [1] supporting open access to most of its microscopes (listed above), and implicitly also to its expertise. The portfolio includes, among other imaging equipment located at other departments, a Nipkow spinning-disk (“Carv II“) confocal microscope [2].





2.      Biochemistry of Membrane Receptors

Our research focuses on the cellular and molecular mechanisms of desensitization of hormone response mediated by the activation of G protein coupled receptors (GPCR). G protein coupled receptors are plasma membrane integral proteins which serve as transducers of extracellular signals across the plasma membrane bilayer to the cell interior. GPCR play a key role in the regulation of many physiological processes and functions. Moreover, GPCRs represent one of the most important groups of targets for therapeutics.


The main areas of the research are:

  • The role of cell membrane and membrane domains
  • Opioid receptors and drug addiction
  • The effect of monovalent ions on δ-opioid receptors – analysis of lithium effect in living cells and isolated cell membranes
  • GABA-B - signalling cascades


Detail informations here.




Vošahlíková, Miroslava - Ujčíková, Hana - Hloušková, Martina - Musil, Stanislav - Roubalová, Lenka - Alda, M. - Svoboda, Petr . Induction of oxidative stress by long-term treatment of live HEK293 cells with therapeutic concentration of lithium is associated with down-regulation of delta-opioid receptor amount and function . Biochemical Pharmacology 2018, roč. 154, Aug, p. 452-463 . IF = 4.235 [ASEP] [ doi ]
Vetrík, Miroslav - Pařízek, Martin - Hadraba, Daniel - Kukačková, Olivia - Brus, Jiří - Hlídková, Helena - Kománková, Lucie - Hodan, Jiří - Sedláček, Ondřej - Šlouf, Miroslav - Bačáková, Lucie - Hrubý, Martin . Porous heat-treated polyacrylonitrile scaffolds for bone tissue engineering . ACS Applied Materials and Interfaces 2018, roč. 10, 10, p. 8496-8506 . IF = 8.097 [ASEP] [ doi ]
Prats-Galino, A. - Čapek, Martin - Reina, M. A. - Cvetko, E. - Radochová, Barbora - Tubbs, R.S. - Damjanovska, M. - Pintaric, T.S. 3D reconstruction of peripheral nerves from optical projection tomography images: A method for studying fascicular interconnections and intraneural plexuses . Clinical Anatomy 2018, roč. 31, 3, p. 424-431 . IF = 1.908 [ASEP] [ doi ]
Pajorová, Julie - Bačáková, Markéta - Musílková, Jana - Brož, Antonín - Hadraba, Daniel - Lopot, F. - Bačáková, Lucie . Morphology of a fibrin nanocoating influences dermal fibroblast behavior . International Journal of Nanomedicine 2018, roč. 13, June, p. 3367-3380 . IF = 4.370 [ASEP] [ doi ]
Meznaric, M. - Eržen, I. - Karen, Petr - Cvetko, E. Effect of ageing on the myosin heavy chain composition of the human sternocleidomastoid muscle . Annals of Anatomy-Anatomischer Anzeiger 2018, roč. 216, March, p. 95-99 . IF = 1.852 [ASEP] [ doi ]

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Institute of Physiology CAS
Department of Biomathematics

Vídeňská 1083
14220 Prague 4

tel.    +420 241 062 768

tel.    +420 241 062 478


RNDr. Jiří Janáček, PhD.
Head of department
RNDr. Lucie Kubínová, CSc.
Deputy head of department
Ing. Martin Čapek, PhD.
Senior researcher (part-time)
RNDr. Barbora Radochová, PhD.
Senior researcher
Ing. Mgr. Daniel Hadraba, Ph.D.
Postdoctoral researcher
MUDr. Petr Karen, CSc.
Senior researcher

Mgr. David Vondrášek


Mgr. Zuzana Kubínová


Ing. Jitka Čočková
Project manager
Dr. Pavla Chotěborská


Biochemistry of Membrane Receptors

Doc. RNDr. Petr Svoboda, DrSc.
Senior researcher
RNDr. Lenka Roubalová, PhD.
Senior researcher
RNDr. Hana Ujčíková, PhD.
Senior researcher
Ing. Miroslava Vošahlíková, PhD.
Senior researcher
Mgr. Jana Brejchová, PhD.
Senior researcher
  Mgr. Kristina Cechová
PhD student