Intranet Čeština

Biomathematics

Biomathematics

The Department of Biomathematics consists of two research groups: (1) Bioimaging and Image Analysis, and (2) Biochemistry of Membrane Receptors. Operating mainly at in situ and in vitro levels, respectively, the two groups complement each other when studying phenomena confined to the cell membrane.

 

1.      Bioimaging and Image Analysis

The group conducts research into the 3D microanatomical aspects of physiological phenomena at mesoscopic, microscopic and ultrastructural level. It is engaged in a range of collaborative projects across the campus and beyond. If interested please contact us (jiri.janacek@fgu.cas.cz).

 

CURRENTLY FUNDED PROJECTS

  •  Brain development in birds, as relevant to the ability to fly
  •  The conduction system of developing murine heart

 

LONG-TERM PROJECTS

  • Development of 3D image analysis and stereological methods including software (employed, e.g., in a volumetric analysis of Langerhans islets from human donors)
  • Microscopic optical-contrasting (label-free) strategies to visualize living cells and tissues

 

OTHER PROJECTS (selection only)

  • Effects of ionizing radiation on blood capillaries network
  • Effects of elevated CO2 on the photosynthetic apparatus’ performance and  ultrastructure
  • Mechanisms governing the formation of extracellular matrix proteins (collagen and elastin)

 

IN-HOUSE IMAGING MODALITIES

  • Laser scanning confocal microscopy
  • Two-photon excitation microscopy
  • Second harmonic generation (SHG) microscopy
  •  ‘White-light‘ laser and hybrid detectors (on Leica TCS SP8 WLL MP microscope)
  • FRAP recording, FRET analysis, FLIM/PLIM recording including λ2 scans 
  • Optical projection tomography (OPT)
  • Selective plane illumination (light sheet) microscopy (SPIM)
  • Phase-, modulation- and interference contrast microscopy

 

AVAILABLE SOFTWARE AND PROTOCOLS

  • Software for 3D image analysis, stereology, spatial statistics and deconvolution (Hyughens)
  • Optical/chemical clearing protocols for OPT and SPIM

 

OPEN ACCESS TO EQUIPMENT

The group is part of the Czech BioImaging infrastructure [1] supporting open access to most of its microscopes (listed above), and implicitly also to its expertise. The portfolio includes, among other imaging equipment located at other departments, a Nipkow spinning-disk (“Carv II“) confocal microscope [2].

[1]      http://www.czech-bioimaging.cz/

[2]      http://www.fgu.cas.cz/en/articles/529-czech-bioimaging-2016-2019

 

 

2.      Biochemistry of Membrane Receptors

Our research focuses on the cellular and molecular mechanisms of desensitization of hormone response mediated by the activation of G protein coupled receptors (GPCR). G protein coupled receptors are plasma membrane integral proteins which serve as transducers of extracellular signals across the plasma membrane bilayer to the cell interior. GPCR play a key role in the regulation of many physiological processes and functions. Moreover, GPCRs represent one of the most important groups of targets for therapeutics.

 

The main areas of the research are:

  • The role of cell membrane and membrane domains
  • Opioid receptors and drug addiction
  • The effect of monovalent ions on δ-opioid receptors – analysis of lithium effect in living cells and isolated cell membranes
  • GABA-B - signalling cascades

 

Detail informations here.

Projects

Achievements

Publications

Wilhelm, Jiří - Vytášek, Richard - Uhlík, Jiří - Vajner, Luděk . Oxidative Stress in the Developing Rat Brain due to Production of Reactive Oxygen and Nitrogen Species . Oxidative Medicine and Cellular Longevity. 2016, roč. 2016, 2016, p. 5057610 . IF = 4.492 [ASEP] [ doi ]
Vošahlíková, Miroslava - Svoboda, Petr . Lithium - therapeutic tool endowed with multiple beneficiary effects caused by multiple mechanisms . Acta Neurobiologiae Experimentalis. 2016, roč. 76, 1, p. 1-19 . IF = 1.708 [ASEP]
Ujčíková, Hana - Vošahlíková, Miroslava - Roubalová, Lenka - Svoboda, Petr . Proteomic analysis of protein composition of rat forebrain cortex exposed to morphine for 10 days; comparison with animals exposed to morphine and subsequently nurtured for 20 days in the absence of this drug . Journal of Proteomics. 2016, roč. 145, Aug 11, p. 11-23 . IF = 3.867 [ASEP] [ doi ]
Tonar, Z. - Tomášek, P. - Loskot, P. - Janáček, Jiří - Králíčková, M. - Witter, K. Vasa vasorum in the tunica media and tunica adventitia of the porcine aorta . Annals of Anatomy-Anatomischer Anzeiger. 2016, roč. 205, 2016, p. 22-36 . IF = 1.308 [ASEP] [ doi ]
Techniková, L. - Tunák, M. - Janáček, Jiří . Pilling evaluation of patterned fabrics based on a gradient field method . Indian Journal of Fibre & Textile Research. 2016, roč. 41, 1, p. 97-101 . IF = 0.420 [ASEP]

Article photogallery

Contacts

Institute of Physiology CAS
Department of Biomathematics

Vídeňská 1083
14220 Prague 4
tel. +420 241 062 768
 

jiri.janacek@fgu.cas.cz

 

tel.    +420 241 062 478

svobodap@fgu.cas.cz

People

  RNDr. Jiří Janáček, PhD.
Head of department
  RNDr. Lucie Kubínová, CSc.
Deputy head of department
  Ing. Martin Čapek, PhD.
Senior researcher (part-time)
  RNDr. Radek Pelc, DPhil.
Senior researcher
  RNDr. Barbora Radochová, PhD.
Senior researcher
  Alexandr Čerňavský, PhD.
Postdoctoral researcher
  Mgr. Daniel Hadraba
PhD student
  MUDr. Petr Karen, CSc.
Senior researcher (part-time)
  Ing. Kateřina Špačková
Project manager
  Alena Dědičová
Technician (part-time)
 

 

Biochemistry of Membrane Receptors

  Doc. RNDr. Petr Svoboda, DrSc.
Group leader
  RNDr. Lenka Roubalová, PhD.
Senior researcher
  RNDr. Hana Ujčíková, PhD.
Senior researcher
  Ing. Miroslava Vošahlíková, PhD.
Senior researcher
  Mgr. Jana Brejchová, PhD.
Senior researcher
  Ing. Kateřina Stolařová
PhD student
  Mgr. Martina Hloušková
PhD student
  Mgr. Kristina Cechová
PhD student
  Mgr. Michaela Czerneková
PhD student
   
   
   
   
   

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