Intranet Čeština

Cellular Neurophysiology

Cellular Neurophysiology Electrophysiological recording of glutamate-induced current responses in cultured hippocampal neurons.

Our research is mainly directed to exploration of functional and pharmacological properties of ion channels, including those activated during synaptic transmission. For that purpose, we use electrophysiological methods, primarily patch clamp technique, combined with molecular-biology, immunohistochemical and microfluorometric methods (Ca2+ imaging). Using this approach, we study properties of excitable cells, such as cultured hippocampal and granular neurons or in vitro preserved neural circuits of pyramidal neurons in the hippocampal brain slices, as well as HEK293 and COS cell lines transfected with recombinant NMDA or vanilloid receptors. The goal of our research is to comprehend the relationship between structure and function of ion channels, to characterize the actions of pharmacologically active substances and to explain the molecular mechanism by which they influence ligand-gated ion channels.

For more detailed understanding of physiological mechanisms and pharmacology of ligand-gated ion channels, we use quantitative analytical techniques (including the analysis of single ion channels, measuring of intracellular concentrations of Ca2+ and mathematical modeling).


For more information and current projects visit department´s web.


Vyklický, Vojtěch - Smejkalová, Tereza - Krausová, Barbora - Balík, Aleš - Kořínek, Miloslav - Borovská, Jiřina - Horák, Martin - Chvojková, Markéta - Kletečková, Lenka - Valeš, Karel - Černý, Jiří - Nekardová, Michaela - Chodounská, Hana - Kudová, Eva - Vyklický ml., Ladislav . Preferential Inhibition of Tonically over Phasically Activated NMDA Receptors by Pregnane Derivatives . Journal of Neuroscience. 2016, roč. 36, 7, p. 2161-2175 . IF = 5.924 [ASEP] [ doi ]
Slavíková, Barbora - Chodounská, Hana - Nekardová, Michaela - Vyklický, Vojtěch - Ladislav, Marek - Hubálková, Pavla - Krausová, Barbora - Vyklický ml., Ladislav - Kudová, Eva . Neurosteroid-like Inhibitors of N-Methyl-D-aspartate Receptor: Substituted 2-Sulfates and 2-Hemisuccinates of Perhydrophenanthrene . Journal of Medicinal Chemistry. 2016, roč. 59, 10, p. 4724-4739 . IF = 5.589 [ASEP] [ doi ]
Kaniaková, Martina - Lichnerová, Katarina - Skřenková, Kristýna - Vyklický ml., Ladislav - Horák, Martin . Biochemical and electrophysiological characterization of N-glycans on NMDA receptor subunits . Journal of Neurochemistry. 2016, roč. 138, 4, p. 546-556 . IF = 3.842 [ASEP] [ doi ]
Jirků, Michaela - Lánský, Zdeněk - Bednárová, L. - Šulc, Miroslav - Monincová, L. - Majer, P. - Vyklický ml., Ladislav - Vondrášek, J. - Teisinger, Jan - Boušová, Kristýna . The characterization of a novel S100A1 binding site in the N-terminus of TRPM1 . International Journal of Biochemistry and Cell Biology. 2016, roč. 78, Sep 2016, p. 186-193 . IF = 3.905 [ASEP] [ doi ]
Hynková, Anna - Maršáková, Lenka - Vašková, Jana - Vlachová, Viktorie . N-terminal tetrapeptide T/SPLH motifs contribute to multimodal activation of human TRPA1 channel . Scientific Reports. 2016, roč. 6, Jun 27, p. 28700 . IF = 5.228 [ASEP] [ doi ]

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Institute of Physiology AS CR, v.v.i.
Department of Cellular Neurophysiology

Vídeňská 1083
14220 Prague 4
tel. +420 241 062 450
fax. +420 241 062 488


prof. MUDr. Ladislav Vyklický, DrSc.
head of the department
RNDr. Viktorie Vlachová, DrSc.
deputy head of the department
RNDr. Aleš Balík, Ph.D.
senior researcher
Ing. Ivan Dittert, Ph.D.
senior researcher
Mgr. Martin Horák, Ph.D.
senior researcher
Mgr. Miloslav Kořínek, Ph.D.
vědecký pracovník
RNDr. Jan Krůšek, Ph.D.
senior researcher
Prof. RNDr. František Vyskočil, DrSc.
senior researcher
Mgr. Martina Kaniaková, Ph.D.
RNDr. Lenka Vyklická, Ph.D.
Tereza Smejkalová, Ph.D.
RNDr. Vojtěch Vyklický, Ph.D.
Mgr. Lucie Zímová, Ph.D.
Mgr. Anna Kádková (b. Hynková)
PhD student
Mgr. Barbora Krausová
PhD student
Mgr. Katarina Lichnerová
PhD student
Mgr. Filip Touška
PhD student
Mgr. Marek Ladislav
PhD student
Ing. Pavla Hubálková
PhD student
Mgr. Kristýna Skřenková
PhD student
Jiří Kaucký
Miloslava Kuldová
Magda Kuntošová