Intranet Čeština

Cellular Neurophysiology

Cellular Neurophysiology Electrophysiological recording of glutamate-induced current responses in cultured hippocampal neurons.

Our research is mainly directed to exploration of functional and pharmacological properties of ion channels, including those activated during synaptic transmission. For that purpose, we use electrophysiological methods, primarily patch clamp technique, combined with molecular-biology, immunohistochemical and microfluorometric methods (Ca2+ imaging). Using this approach, we study properties of excitable cells, such as cultured hippocampal and granular neurons or in vitro preserved neural circuits of pyramidal neurons in the hippocampal brain slices, as well as HEK293 and COS cell lines transfected with recombinant NMDA or vanilloid receptors. The goal of our research is to comprehend the relationship between structure and function of ion channels, to characterize the actions of pharmacologically active substances and to explain the molecular mechanism by which they influence ligand-gated ion channels.

For more detailed understanding of physiological mechanisms and pharmacology of ligand-gated ion channels, we use quantitative analytical techniques (including the analysis of single ion channels, measuring of intracellular concentrations of Ca2+ and mathematical modeling).


For more information and current projects visit department´s web.


Vyklická, Lenka - Boukalová, Štěpána - Mačíková, Lucie - Chvojka, Štěpán - Vlachová, Viktorie . The human transient receptor potential vanilloid 3 channel is sensitized via the ERK pathway . Journal of Biological Chemistry.. 2017, roč. 292, 51, p. 21083-21091 . IF = 4.125 [ASEP] [ doi ]
Touška, Filip - Sattler, S. - Malsch, P. - Lewis, R. J. - Reeh, P. W. - Zimmermann, K. Ciguatoxins Evoke Potent CGRP Release by Activation of Voltage-Gated Sodium Channel Subtypes Na(V)1.9, Na(V)1.7 and Na(V)1.1 . Marine Drugs. 2017, roč. 15, 9, článku 269 . IF = 3.503 [ASEP] [ doi ]
Petrovič, Miloš - da Silva, S. V. - Clement, J. P. - Vyklický ml., Ladislav - Mulle, C. - González-González, I. M. - Henley, J. M. Metabotropic action of postsynaptic kainate receptors triggers hippocampal long-term potentiation . Nature Neuroscience. 2017, roč. 20, 4, p. 529-539 . IF = 17.839 [ASEP] [ doi ]
Maršáková, Lenka - Barvík, I. - Zíma, V. - Zímová, Lucie - Vlachová, Viktorie . The First Extracellular Linker Is Important for Several Aspects of the Gating Mechanism of Human TRPA1 Channel . Frontiers in Molecular Neuroscience. 2017, roč. 10, Jan 31, článku 16 . IF = 5.076 [ASEP] [ doi ]
Kádková, Anna - Synytsya, Viktor - Krůšek, Jan - Zímová, Lucie - Vlachová, Viktorie . Molecular Basis of TRPA1 Regulation in Nociceptive Neurons. A Review . Physiological Research. 2017, roč. 66, 3, p. 425-439 . IF = 1.461 [ASEP]

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Institute of Physiology AS CR, v.v.i.
Department of Cellular Neurophysiology

Vídeňská 1083
14220 Prague 4
tel. +420 241 062 450
fax. +420 241 062 488


prof. MUDr. Ladislav Vyklický, DrSc.
head of the department
RNDr. Viktorie Vlachová, DrSc.
deputy head of the department
RNDr. Aleš Balík, Ph.D.
senior researcher
Ing. Ivan Dittert, Ph.D.
senior researcher
Mgr. Martin Horák, Ph.D.
senior researcher
Mgr. Miloslav Kořínek, Ph.D.
vědecký pracovník
RNDr. Jan Krůšek, Ph.D.
senior researcher
Prof. RNDr. František Vyskočil, DrSc.
senior researcher
Mgr. Martina Kaniaková, Ph.D.
RNDr. Lenka Vyklická, Ph.D.
Tereza Smejkalová, Ph.D.
RNDr. Vojtěch Vyklický, Ph.D.
Mgr. Lucie Zímová, Ph.D.
Mgr. Anna Kádková (b. Hynková)
PhD student
Mgr. Barbora Krausová
PhD student
Mgr. Katarina Lichnerová
PhD student
Mgr. Filip Touška
PhD student
Mgr. Marek Ladislav
PhD student
Ing. Pavla Hubálková
PhD student
Mgr. Kristýna Skřenková
PhD student
Jiří Kaucký
Miloslava Kuldová
Magda Kuntošová