Intranet Čeština



We study the physiology, biochemistry and pharmacology of cholinergic neurons at molecular level. In our study we mainly employ cell lines but we also use animal models. Our research is focused mainly on these topics:


  • Biochemical physiology and pharmacology of cholinergic neurons. Development and differentiation of cholinergic neurons. Synthesis, storage, and release of acetylcholine. Presynaptic regulation of acetylcholine release.
  • Cholinergic mechanisms in pathogenesis of Alzheimer´s disease. Influence of beta-amyloid on acetylcholine metabolism and muscarinic transmission.
  • Molecular pharmacology of muscarinic receptors. Allosteric modulation of receptor activation. Interaction of muscarinic receptors with G-proteins. Modeling of muscarinic receptor signal transduction.



Book: Muscarinic Receptor: From Structure to Animal Models

Dr. Jan Jakubik Institute of Physiology Academy of Sciences and professor. Jaromir Mysliveček Institute of Physiology 1st Faculty of Medicine were approached to write the current list of methods that are used in research of muscarinic receptor as part of a Neuromethods series published by Springer. More


Lyukmanova, E. N. - Shulepko, M. A. - Shenkarev, Z. O. - Bychkov, M. L. - Paramonov, A. S. - Chugunov, A. O. - Kulbatskii, D. S. - Arvaniti, M. - Dolejší, Eva - Schaer, T. - Arseniev, A. S. - Efremov, R. G. - Thomsen, M. S. - Doležal, Vladimír - Bertrand, D. - Dolgikh, D. A. - Kirpichnikov, M. P. Secreted Isoform of Human Lynx1 (SLURP-2): Spatial Structure and Pharmacology of Interactions with Different Types of Acetylcholine Receptors . Scientific Reports. 2016, roč. 6, Aug 3, p. 30698 . IF = 5.228 [ASEP] [ doi ]
Koivisto, H. - Leinonen, H. - Puurula, M. - Hafez, H. S. - Barrera Alquicer, Glenda - Stridh, M. L. - Waagepetersen, H. S. - Tiainen, M. - Soininen, P. - Zilberter, Y. - Tanila, H. Chronic Pyruvate Supplementation Increases Exploratory Activity and Brain Energy Reserves in Young and Middle-Aged Mice . Frontiers in Aging Neuroscience. 2016, roč. 8, Mar 16, p. 41 . IF = 4.348 [ASEP] [ doi ]
Dolejší, Eva - Liraz, O. - Rudajev, Vladimír - Zimčík, Pavel - Doležal, Vladimír - Michaelson, D. M. Apolipoprotein E4 reduces evoked hippocampal acetylcholine release in adult mice . Journal of Neurochemistry. 2016, roč. 136, 3, p. 503-509 . IF = 3.842 [ASEP] [ doi ]
Roubalová, Lenka - Vošahlíková, Miroslava - Brejchová, Jana - Sýkora, Jan - Rudajev, Vladimír - Svoboda, Petr . High Efficacy but Low Potency of delta-Opioid Receptor-G Protein Coupling in Brij-58-Treated, Low-Density Plasma Membrane Fragments . PLoS ONE. 2015, Vol. 10, 8, e0135664 . IF = 3.057 [ASEP] [ doi ]
Randáková, Alena - Dolejší, Eva - Rudajev, Vladimír - Zimčík, Pavel - Doležal, Vladimír - El-Fakahany, E. E. - Jakubík, Jan . Classical and atypical agonists activate M1 muscarinic acetylcholine receptors through common mechanisms . Pharmacological Research. 2015, Vol. 97, Jul 2015, p. 27-39 . IF = 4.816 [ASEP] [ doi ]

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Institute of Physiology AS CR, v.v.i.
Department of Neurochemistry

Vídeňská 1083
14220 Prague 4
tel. +420 241 062 620
fax. +420 241 062 488


  Mgr. Jan Jakubík, Ph.D.
head of the department
  MUDr. Vladimír Doležal, DrSc.
deputy head of the department
  RNDr. Vladimír Rudajev Ph.D.
senior researcher
  MUDr. et Mgr. Helena Janíčková, Ph.D.
postdoc (intern at UWO, Canada)
  Mgr. Eva Machová, Ph.D.
postdoc (maternity leave)
  Mgr. Alena Randáková, Ph.D.
postdoc (intern at FAU, Germany)
  Mgr. Eva Dolejší, Ph.D.
postdoc (maternity leave)
  Romana Ondřejová
  Dana Ungerová