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Protein Structures

Protein Structures

http://www.biomed.cas.cz/d312/index.php?section=home

Our group is focused on structural biology (the relationship between the structure and function of certain groups of proteins), particularly we focus on the proteins which participate in the signal transmission in the cell. Among methods we use are recombinant protein expression, biophysical characterization, study of intermolecular interactions, protein structure and interaction surfaces. All these methods enable us to better understand the details how the activity and function of protein-protein complexes is regulated. Our research is focused mainly on:

 

  • Structural biology of 14-3-3 proteins and their complexes
  • Mechanism of regulation of proteinkisases CaMKK1, CaMKK2 and ASK1
  • Study of inhibition of protease caspase-2 by 14-3-3 protein
  • Study of DNA-binding domain of transcription factor FOXO4

Projects

PIP2 and PIP3 interact with N-terminus region of TRPM4 channel

Transient receptor potential (TRP) channels are a wide family of non-selective ion channels responsible for monovalent and divalent cation influx into the cells. Members of this family are involved in many sensory processes. We identified two positively charged residues as having a crucial impact on PIP2/PIP3 binding.  More

Achievements

Tomáš Obšil was awarded the title of professor

Prof. RNDr. Tomáš Obšil, Ph.D. was awarded by the title of professor at The Faculty of Science, Charles University, majoring in Physical Chemistry. Professors graduation will take place December 18, 2014. More

Publications

Petrvalská, Olivia - Košek, Dalibor - Kukačka, Zdeněk - Tošner, Z. - Man, Petr - Večeř, J. - Herman, P. - Obšilová, Veronika - Obšil, Tomáš . Structural Insight into the 14-3-3 Protein-dependent Inhibition of Protein Kinase ASK1 (Apoptosis Signal-regulating kinase 1) . Journal of Biological Chemistry. 2016, roč. 291, 39, p. 20753-20765 . IF = 4.258 [ASEP] [ doi ]
Kylarová, Salome - Košek, Dalibor - Petrvalská, Olivia - Pšenáková, Katarína - Man, Petr - Večeř, J. - Herman, P. - Obšilová, Veronika - Obšil, Tomáš . Cysteine residues mediate high‐affinity binding of thioredoxin to ASK1 . FEBS Journal. 2016, roč. 283, 20, p. 3821-3838 . IF = 4.237 [ASEP] [ doi ]
Kirubakaran, P. - Pfeiferová, L. - Boušová, Kristýna - Bednárová, L. - Obšilová, Veronika - Vondrášek, J. Artificial proteins as allosteric modulators of PDZ3 and SH3 in two-domain constructs: A computational characterization of novel chimeric proteins . Proteins-Structure, Function and Bioinformatics. 2016, roč. 84, 10, p. 1358-1374 . IF = 2.499 [ASEP] [ doi ]
Jirků, Michaela - Lánský, Zdeněk - Bednárová, L. - Šulc, Miroslav - Monincová, L. - Majer, P. - Vyklický ml., Ladislav - Vondrášek, J. - Teisinger, Jan - Boušová, Kristýna . The characterization of a novel S100A1 binding site in the N-terminus of TRPM1 . International Journal of Biochemistry and Cell Biology. 2016, roč. 78, Sep 2016, p. 186-193 . IF = 3.905 [ASEP] [ doi ]
Jirků, Michaela - Bumba, Ladislav - Bednárová, Lucie - Kubala, M. - Šulc, Miroslav - Franěk, M. - Vyklický ml., Ladislav - Vondrášek, Jiří - Teisinger, Jan - Boušová, Kristýna . Characterization of the part of N-terminal PIP2 binding site of the TRPM1 channel . Biophysical Chemistry. 2015, Vol. 207, Dec, p. 135-142 . IF = 2.363 [ASEP] [ doi ]

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Contacts

Institute of Physiology AS CR, v.v.i.
Department of Protein Structures

Vídeňská 1083
14220 Prague 4
tel. +420 241 062 191
fax. +420 241 062 477
 

veronika.obsilova@fgu.cas.cz

People

 RNDr. Veronika Obšilová, Ph.D.
 head of the department
Prof. RNDr. Tomáš Obšil, Ph.D.
deputy head of department

RNDr. Miroslava Alblová (born Kopecká), Ph.D.
junior researcher

Ing. Jan Teisinger, CSc.
senior researcher

M.Sc. Kristýna Boušová, Ph.D.
junior researcher

Ing. Michaela Jirků
PhD student
Mgr. Salome Kylarová
PhD student
Mgr. Dana Kalábová (born Žáková) 
PhD student
Mgr. Aneta Šmídová
PhD student
Mgr. Olívia Petrvalská
PhD student

Mgr. Katarína Pšenáková
PhD student

Bc. Kateřina Jarosilová
undergraduate student
Klára Kohoutová
undergraduate student
  Nicola Koupilová
pregraduální student
Mgr. Linda Malá
technician
Markéta Kolcunová
technician
   

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