Intranet Čeština

Structural Biology of Signaling Proteins

Structural Biology of Signaling Proteins

Our group is focused on structural biology (the relationship between the structure and function of certain groups of proteins), particularly we focus on the proteins which participate in the signal transmission in the cell. Among methods we use are recombinant protein expression, biophysical characterization, study of intermolecular interactions, protein structure and interaction surfaces. All these methods enable us to better understand the details how the activity and function of protein-protein complexes is regulated. Our research is focused mainly on:


  • Structural biology of 14-3-3 proteins and their complexes
  • Mechanism of regulation of proteinkisases CaMKK1, CaMKK2 and ASK1
  • Study of inhibition of protease caspase-2 by 14-3-3 protein
  • Study of DNA-binding domain of transcription factor FOXO4



Tomáš Obšil was awarded the title of professor

Prof. RNDr. Tomáš Obšil, Ph.D. was awarded by the title of professor at The Faculty of Science, Charles University, majoring in Physical Chemistry. Professors graduation will take place December 18, 2014. More


Kalábová, Dana - Šmídová, Aneta - Petrvalská, Olivia - Alblová, Miroslava - Košek, Dalibor - Man, Petr - Obšil, Tomáš - Obšilová, Veronika . Human procaspase-2 phosphorylation at both S139 and S164 is required for 14-3-3 binding . Biochemical and Biophysical Research Communications. 2017, roč. 493, 2, p. 940-945 . IF = 2.559 [ASEP] [ doi ]
Kacířová, Miroslava - Nováček, J. - Man, Petr - Obšilová, Veronika - Obšil, Tomáš . Structural Basis for the 14-3-3 Protein-Dependent Inhibition of Phosducin Function . Biophysical Journal. 2017, roč. 112, 7, p. 1339-1349 . IF = 3.495 [ASEP] [ doi ]
Alblová, Miroslava - Šmídová, Aneta - Dočekal, V. - Veselý, J. - Herman, P. - Obšilová, Veronika - Obšil, Tomáš . Molecular basis of the 14-3-3 protein-dependent activation of yeast neutral trehalase Nth1 . Proceedings of the National Academy of Sciences of the United States of America. 2017, roč. 114, 46, E9811-E9820 . IF = 9.504 [ASEP] [ doi ]
Kirubakaran, P. - Pfeiferová, L. - Boušová, Kristýna - Bednárová, L. - Obšilová, Veronika - Vondrášek, J. Artificial proteins as allosteric modulators of PDZ3 and SH3 in two-domain constructs: A computational characterization of novel chimeric proteins . Proteins-Structure, Function and Bioinformatics. 2016, roč. 84, 10, p. 1358-1374 . IF = 2.397 [ASEP] [ doi ]
Petrvalská, Olivia - Košek, Dalibor - Kukačka, Zdeněk - Tošner, Z. - Man, Petr - Večeř, J. - Herman, P. - Obšilová, Veronika - Obšil, Tomáš . Structural Insight into the 14-3-3 Protein-dependent Inhibition of Protein Kinase ASK1 (Apoptosis Signal-regulating kinase 1) . Journal of Biological Chemistry. 2016, roč. 291, 39, p. 20753-20765 . IF = 4.125 [ASEP] [ doi ]

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Institute of Physiology AS CR, v.v.i.
Department of Structural Biology of Signaling Proteins 

detached workplace: BIOCEV,
Průmyslová 595, 252 50 Vestec

+420 325 873 513


 RNDr. Veronika Obšilová, Ph.D.
 head of the department
Prof. RNDr. Tomáš Obšil, Ph.D.
deputy head of department

RNDr. Miroslava Alblová (born Kopecká), Ph.D.
junior researcher

Mgr. Matěj Horváth, Ph.D.
junior researcher
Mgr. Salome Kylarová
PhD student
Mgr. Dana Kalábová (born Žáková) 
PhD student
Mgr. Aneta Šmídová
PhD student
Mgr. Olívia Petrvalská
PhD student

RNDr. Katarína Pšenáková
PhD student

Bc. Karolína Honzejková
undergraduate student
Klára Kohoutová
undergraduate student
Nicola Koupilová
undergraduate student
Martina Mikulů
undergraduate student
Mgr. Linda Malá
Markéta Kolcunová