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LECTURE "Mitochondrial metabolic rewiring during the developmental differentiation of Trypanosoma brucei"


Throughout the Trypanosoma brucei life cycle, the parasite´s single mitochondrion undergoes extensive structural and metabolic rewiring as it transforms from an ATP-producing organelle in the insect form to ATP-consuming organelle in the mammalian form. Complex mitochondrial remodeling was examined using –omics and traditional biochemical methods. Our data showed that during the parasite´s differentiation i) electron flow from OXPHOS pathway is redirected to alternative oxidase; ii) ATP levels are decreased; iii) respiratory complexes III and IV are progressively down-regulated iv) activity of certain TCA cycle enzymes and complex II are increased; v) ROS molecules are elevated. Importantly, exogenous expression of catalase, a ROS scavenger, severely limits cellular differentiation, suggesting that ROS are acting as a retrograde signal. Our findings provide insights into the emerging concept that mitochondria act as signaling organelles through the release of TCA cycle derived metabolites and ROS to drive cellular differentiation.

Výsledek obrázku pro RNDr. Alena Panicucci Zíková, Ph.D.,

 

Kontaktní osoba v FGÚ: Tomáš Mráček, tomas.mracek@fgu.cas.cz