Department: Functional Morphology
Research Summary: The main research interest of our department is to study mechanisms of pain and to explore new possibilities of pain treatment, especially in chronic states. Our experimental work is concentrated on the modulation of nociceptive information at the spinal cord level that is the relay center between the periphery and higher brain areas. Our goal is to study these modulatory mechanisms in order to improve therapy for pain conditions, such as allodynia, hyperalgesia, neuropathic and cancer related pain. Our focus is to study modulation of synaptic transmission at the spinal cord level. Lately we are interested in the role of TRPV1 receptors, cannabinoids, opioids and inflammatory cytokines in this process. In our research we use mainly electrophysiological, imaging, immunohistochemical and behavioral methods.
Modulation of nociceptive signaling at spinal cord level.
We are highly interested in different aspects of spinal TRPV1 receptors modulation of nociceptive synaptic transmission at the spinal cord level. This project will be focused on their interaction with other types of receptors especially under pathological conditions. Electrophysiological and imaging recordings, molecular biology, immunohistochemical and behavioral methods will be used to study different models of pathological pain.
Candidate Requirements: Candidates should have a Masters' degree in biological, medical or chemical sciencess, or be due to complete their studies in this academic year. Experience in cell biology, molecular biology or electrophysiology techniques would be an advantage.
D. Spicarova, P. Adamek, N. Kalynovska, P. Mrozkova, J. Palecek, TRPV1 receptor inhibition decreases CCL2-induced hyperalgesia. Neuropharmacology 81:75-84, 2014 IF=4.82
Uchytilová,E - Špicarová,D - Paleček,J. TRPV1 antagonist attenuates postoperative hypersensitivity by central and peripheral mechanisms. Molecular Pain 2014, 10, 2014, p. 67. IF = 3.654
Li Y, Adamek P, Zhang H, Tatsui CE, Rhines LD, Mrozkova P, Li Q, Kosturakis AK, Cassidy RM, Harrison.DS, Cata,J.P. Sapire,K. Zhang,H. Kennamer, R. M. Jawad, A.B. - Ghetti, A. - Yan, J. - Paleček, J. Dougherty, P. M. The Cancer Chemotherapeutic Paclitaxel Increases Human and Rodent Sensory Neuron Responses to TRPV1 by Activation of TLR4. J Neurosci. 2015;35(39):13487-13500. IF=6.34
Uchytilová, E - Špicarová, D - Paleček, J. Single high-concentration capsaicin application prevents c-Fos expression in spinothalamic and postsynaptic dorsal column neurons after surgical incision . European Journal of Pain. 2015, Vol. 19, 10, p. 1496-1505 . IF = 2.9
Mrózková,P, Špicarová,D - Paleček, J. Hypersensitivity Induced by Activation of Spinal Cord PAR2 Receptors Is Partially Mediated by TRPV1 Receptors. PLoS ONE. 2016,11, 10, e0163991 . IF = 3.057
Nerandzic V, Mrozkova P, Adamek P, Spicarova D, Nagy I, Palecek J. Peripheral inflammation affects modulation of nociceptive synaptic transmission in the spinal cord induced by N-arachidonoylphosphatidylethanolamine. Br J Pharmacol. 2017 May 5. doi: 10.1111/bph.13849. [Epub ahead of print] IF=5.4
Torres-Pérez JV, Adamek P, Palecek J, Vizcaychipi M, Nagy I, Varga A. The NAv1.7 blocker protoxin II reduces burn injury-induced spinal nociceptive processing. J Mol Med (Berl). 2017 Oct 23. doi: 10.1007/s00109-017-1599-0. [Epub ahead of print] IF=4.7
Supervisor: Jiri Palecek, M.D., Ph.D.