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INSTITUTE OF PHYSIOLOGY CAS

Cutting-edge science for health

Mass spectrometer for metabolomics QTRAP 5500


The instrument is dedicated to metabolomics / lipidomics (small molecules).
 

Specs

- LC-MS/MS system

- mass spectrometer QTRAP 5500 from AB SCIEX

- liquid chromatography Ultimate 3000 RSLC from Dionex/Thermo

- ion source Turbo V (ESI a APCI)

- scans: Q1 MS, Q3 MS, Product Ion, Precursor Ion, Neutral Loss or Gain, MRM, EMS, EPI, ER, MS3, MRM3, TripleTrap

 

Methods

- Kvantitative analysis of oxylipins – eicosanoids, protectins, resolvins, oxidized fatty acids

- Kvantitative analysis of cholesteryl esters – free cholesterol + C2 – C26:x esters

- Kvantitative analysis of steroids

- Kvantitative analysis of acylcarnitines and amino acids (FIA or HILIC, kit Chromsystems)

- Kvantitative analysis of drugs in plasma (metformin, glitazones,...)

- Kvantitative analysis of  glycerophospholipids (shotgun or LC-MS/MS) - PC, PE, PS, PG, PI, lyso*...

- Kvantitative analysis of endocannabinoids - anandamide, 2-arachidonoyl glycerol,...

- Kvalitative analysis of triacylglycerols

 

Contact:  Ondrej Kuda, Ph.D., tel +420 296 443 707 office, +420 296 442 816 lab QTRAP, email: ondrej.kuda@fgu.cas.cz