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Laboratory of Metabolism of Bioactive Lipids

Laboratory of Metabolism of Bioactive Lipids Metabolism of bioactive lipids

The Laboratory focuses on the analysis of lipid mediators (eicosanoids, docosanoids, endocannabinoids) using the UPLC-MS/MS method, in particular to identify the source of production of various mediators and their effect on metabolism and immune cells (i.e. adipocytes vs. macrophages). Currently, the lab is exploring new anti-diabetic lipids, fatty acid esters of hydroxy fatty acids and lipid-related metabolic pathways using stable isotopes and LIMeX platform.

GTTAtlas GTTAtlas - interactive metabolomics and lipidomics atlas of mouse tissues during oral glucose tolerance test (OGTT).

MetaboAtlas21 MetaboAtlas21 - a comprehensive atlas of the mouse metabolome and lipidome.

Projects

Branched fatty acid esters of hydroxy fatty acids (FAHFA)

White adipose tissue (WAT) is a complex endocrine organ and its low-grade inflammation in obesity contributes to the development of metabolic disorders. In 2014, a class of WAT-born lipid mediators - fatty acid esters of hydroxy fatty acids (FAHFA) was discovered. FAHFAs are endogenous lipids with anti-inflammatory and anti-diabetic properties, including the enhancement of glucose tolerance, and insulin and glucagon-like peptide 1 (GLP-1) secretion while reducing inflammatory responses [1-5]. More

Omega-3 polyunsaturated fatty acids

Naturally occurring long-chain omega-3 PUFA, namely eicosapentaenoic acid (EPA; 20:5 ω-3), docosapentaenoic acid (DPA; 22:5 ω-3), and docosahexaenoic acid (DHA; 22:6 ω-3) exert multiple biological effects... More

Open science

Our lab participates in OPEN SCIENCE initiative and hosts high school students with their project in metabolomics. More

Chemoinformatics resources

We run local instances of several services focused on Metabolomics, Lipidomics, Statistical analysis and Chemometrics. As the hardware resources are limited, these tools are available only within campus LAN. More

Local installation of MetaboAnalyst

MetaboAnalyst is a great tool for analysis of metabolomics data. Here are some tips how to install MetaboAnalyst locally on a linux workstation. Such installation could be tailored to meet individual requirements (default setting, fonts, etc.). Starting point is a runnig installation of Ubuntu 18.04.3 LTS. More

CRediT generator

CRediT (Contributor Roles Taxonomy) is high-level taxonomy, including 14 roles, that can be used to represent the roles typically played by contributors to scientific scholarly output. The roles describe each contributor’s specific contribution to the scholarly output. This web tool helps to summarize contributions of individual authors and prepare the Author contributions paragraph for a scientific journal. More

The role of antioxidant defense in the synthesis of antidiabetic lipokines

Obesity is associated with an increased risk of developing metabolic syndrome, type 2 diabetes and cardiovascular disease. The adipose tissue significantly affects the whole-body metabolism of carbohydrates and lipids, which also involves fatty acid esters of hydroxy fatty acids (FAHFA), lipid mediators that affect glucose uptake and insulin sensitivity in target tissues. Our goal is to identify the metabolic pathways between lipid synthesis and antioxidant defense involved in FAHFA biosynthesis, to examine the substrate specifity of the candidate enzyme, and to introduce a methodology for the quantification of metabolic fluxes through redox reactions linking central carbon metabolism and nicotinamidadene cofactor-driven reductive biosynthesis. More

Achievements

Publications

Štěpán; M. - Daďová; K. - Matouš; M. - Krauzová; E. - Sontáková; L. - Koc; M. - Larsen; T. - Kuda; Ondřej - Štich; V. - Rossmeislová; L. - Šiklová; M. Exercise Training Combined with Calanus Oil Supplementation Improves the Central Cardiodynamic Function in Older Women . Nutrients. 2022; 14(1)); 149 . IF = 5.719 [ASEP] [ doi ]
Riečan; Martin - Palůchová; Veronika - Lopes; Magno - Brejchová; Kristýna - Kuda; Ondřej . Branched and linear fatty acid esters of hydroxy fatty acids (FAHFA) relevant to human health . Pharmacology and Therapeutics. 2022; 231(Mar)); 107972 . IF = 12.310 [ASEP] [ doi ]
Fisk; H. L. - Childs; C. E. - Miles; E. A. - Ayres; R. - Noakes; P. S. - Paras-Chavez; C. - Kuda; Ondřej - Kopecký; Jan - Antoun; E. - Lillycrop; K. A. - Calder; P. C. Modification of subcutaneous white adipose tissue inflammation by omega-3 fatty acids is limited in human obesity-a double blind; randomised clinical trial . EBioMedicine. 2022; 77(Mar)); 103909 . IF = 8.143 [ASEP] [ doi ]
Sistilli; Gabriella - Kalendová; Veronika - Čajka; Tomáš - Irodenko; Ilaria - Bardová; Kristina - Oseeva; Marina - Žáček; P. - Kroupová; Petra - Horáková; Olga - Lackner; K. - Gastaldelli; A. - Kuda; Ondřej - Kopecký; Jan - Rossmeisl; Martin . Krill Oil Supplementation Reduces Exacerbated Hepatic Steatosis Induced by Thermoneutral Housing in Mice with Diet-Induced Obesity . Nutrients. 2021; 13(2)); 437 . IF = 5.717 [ASEP] [ doi ]
Lopes; Magno Alves - Brejchová; Kristýna - Riečan; Martin - Nováková; Michaela - Rossmeisl; Martin - Čajka; Tomáš - Kuda; Ondřej . Metabolomics atlas of oral 13C-glucose tolerance test in mice . Cell Reports. 2021; 37(2)); 109833 . IF = 9.423 [ASEP] [ doi ]

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People

Ondrej Kuda, Ph.D.
Head of the Laboratory
Kristyna Brejchova, Ph.D.
Scientist
Ing. Veronika Paluchova
Postgradual Student
Mgr. Martin Riečan
Postgradual Student
Felipe Martínez Ramírez, MSc.
postgradual student
Radka Trubačová, MSc.
labmanager