Metabolism of bioactive lipids
The Laboratory focuses on the analysis of lipid mediators (eicosanoids, docosanoids, endocannabinoids) using the UPLC-MS/MS method, in particular to identify the source of production of various mediators and their effect on metabolism and immune cells (i.e. adipocytes vs. macrophages). Currently, the lab is exploring new anti-diabetic lipids, fatty acid esters of hydroxy fatty acids and lipid-related metabolic pathways using stable isotopes and LIMeX platform.
We are always interested to hear from prospective (MSc, PhD or postdoc) candidates. Please submit a short personal statement detailing your research interests and qualifications, and a CV by email, RE: "metabolomics".
FluxAtlas - interactive metabolomics, lipidomics and fluxomics atlas of mouse tissues during oral glucose tolerance test (OGTT).
MetaboAtlas21 - a comprehensive atlas of the mouse metabolome and lipidome.
Projects
FluxAtlas - interactive metabolomics, lipidomics and fluxomics atlas of mouse tissues during oral glucose tolerance test (OGTT)
Methodological highlighs from publications using small molecule omics techniques.
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Our lab participates in OPEN SCIENCE initiative and hosts high school students with their project in metabolomics.
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White adipose tissue (WAT) is a complex endocrine organ and its low-grade inflammation in obesity contributes to the development of metabolic disorders. In 2014, a class of WAT-born lipid mediators - fatty acid esters of hydroxy fatty acids (FAHFA) was discovered. FAHFAs are endogenous lipids with anti-inflammatory and anti-diabetic properties, including the enhancement of glucose tolerance, and insulin and glucagon-like peptide 1 (GLP-1) secretion while reducing inflammatory responses [1-5].
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We run local instances of several services focused on Metabolomics, Lipidomics, Statistical analysis and Chemometrics. As the hardware resources are limited, these tools are available only within campus LAN.
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Naturally occurring long-chain omega-3 PUFA, namely eicosapentaenoic acid (EPA; 20:5 ω-3), docosapentaenoic acid (DPA; 22:5 ω-3), and docosahexaenoic acid (DHA; 22:6 ω-3) exert multiple biological effects...
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Achievements
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Our laboratory has been awarded the grant GA20-00317S New metabolic pathway in the synthesis of anti-diabetic lipokines (2020-2022).
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Ondrej Kuda has been awarded Lumina Quaeruntur praemium.
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Publications
van der Hoek; M. D. - Nieuwenhuizen; A. G. - Kuda; Ondřej - Bos; P. - Palůchová; Veronika - Verschuren; L. - van den Hoek; A. M. - Kleemann; R. - Veeger; N. J. G. M. - van der Leij; F. R. - Keijer; J.
Intramuscular short-chain acylcarnitines in elderly people are decreased in (pre-)frail females; but not in males
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FASEB Journal. 2020; 34(9); 11658-11671
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IF = 4.966
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Rossmeisl; Martin - Pavlišová; Jana - Bardová; Kristina - Kalendová; Veronika - Burešová; Jana - Kuda; Ondřej - Kroupová; Petra - Staňková; B. - Tvrzická; E. - Fišerová; E. - Horáková; Olga - Kopecký; Jan
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Increased plasma levels of palmitoleic acid may contribute to beneficial effects of Krill oil on glucose homeostasis in dietary obese mice
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Biochimica Et Biophysica Acta-Molecular and Cell Biology of Lipids. 2020; 1865(8)); 158732
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IF = 4.519
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doi
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Pomar; C. A. - Kuda; Ondřej - Kopecký; Jan - Rombaldová; Martina - Castro; H. - Picó; C. - Sánchez; J. - Palou; A.
Maternal diet; rather than obesity itself; has a main influence on milk triacylglycerol profile in dietary obese rats
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Biochimica Et Biophysica Acta-Molecular and Cell Biology of Lipids. 2020; 1865(2)); 158556
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IF = 4.519
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doi
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Palůchová; Veronika - Vik; A. - Čajka; Tomáš - Březinová; Marie - Brejchová; Kristýna - Bugajev; Viktor - Dráberová; Lubica - Dráber; Peter - Burešová; Jana - Kroupová; Petra - Bardová; Kristina - Rossmeisl; Martin - Kopecký; Jan - Hansen; T. V. - Kuda; Ondřej
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Triacylglycerol-Rich Oils of Marine Origin are Optimal Nutrients for Induction of Polyunsaturated Docosahexaenoic Acid Ester of Hydroxy Linoleic Acid (13-DHAHLA) with Anti-Inflammatory Properties in Mice
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Molecular Nutrition & Food Research. 2020; 64(11)); 1901238
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IF = 5.309
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Palůchová; Veronika - Oseeva; Marina - Březinová; Marie - Čajka; Tomáš - Bardová; Kristina - Adamcová; Kateřina - Žáček; Petr - Brejchová; Kristýna - Balas; L. - Chodounská; Hana - Kudová; Eva - Schreiber; R. - Zechner; R. - Durand; T. - Rossmeisl; Martin - Abumrad; N. A. - Kopecký; Jan - Kuda; Ondřej
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Lipokine 5-PAHSA is Regulated by Adipose Triglyceride Lipase and Primes Adipocytes for de novo Lipogenesis in Mice
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Diabetes. 2020; 69(3); 300-312
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IF = 7.720
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Kristyna Magno Ondrej Veronika Marina
Speedvac
Growing cells
QTRAP 5500 / SelexION
Growing cells
Eolid Phase Extraction
