6th  BIOCEV-Krč Methodological Seminar

Topic: In vivo imaging using multiphoton microscopy

Monday 2^nd September 2019 at 15:00 in the Turquoise Lounge (IEM)

Multiphoton microscopy has emerged as a key technique for various applications in the life sciences. It employs near infrared femtosecond lasers to generate observable nonlinear signals in the visible range. This allows it to provide an exceptionally large depth penetration with minimal invasion and photodamage. Multiphoton fluorescence microscopy combines laser scanning microscopy with long-wavelength multiphoton fluorescence excitation to capture three-dimensional images of specimens tagged with specific fluorophores. We use the technique for in vivo imaging of deep-tissue structures such as cellular microstructure in the adipose tissue and for visualization of complex and dynamic biological processes such as sensory-induced neuronal activity.  In this seminar, we will briefly introduce the principles of the method and provide an overview of the relevant instrumentation and techniques available at our Institutes. We will also discuss specific applications of multiphoton microscopy in our labs and present examples of results that we have achieved.

RNDr. Rostislav Tureček, PhD, Institute of Experimental Medicine/Department of Auditory Neuroscience

Introduction to in vivo imaging of neuronal activity using targeted Ca2+ indicators

Ing. Štěpán Kortus, Institute of Experimental Medicine/Microscopy unit

Principles and advantages of multiphoton microscopy. Specifications of the Ultima IV two-photon microscope.

Mgr. Filip Tomáška, Institute of Experimental Medicine/Department of Auditory Neuroscience

In vivo imaging of neuronal activity in the mouse auditory cortex

Ing. Mgr. Daniel Hadraba, Ph.D. Institute of Physiology/Department of Biomathematics

Introduction to Bruker Ultima In-Vivo microscope system

Mgr. David Vondrášek, Institute of Physiology/Department of Biomathematics

Methods and techniques at Bruker Ultima In-Vivo microscope system