Cutting-edge science for health

Beneficial systemic effects of adipose tissue metabolism

Laboratory of Adipose Tissue Biology

PhD project: Beneficial systemic effects of adipose tissue metabolism

In all mammals including humans, two types of adipose tissue are recognized, white and brown adipose tissue (WAT and BAT, respectively), which are engaged in two major distinct and opposite functions: energy storage (in WAT) and energy dissipation (in BAT). Our recent studies are focused on “healthy adipocytes” in WAT, which are relatively small fat cells endowed with a high capacity for mitochondrial oxidative phosphorylation, triacylglycerol/fatty acid (TAG/FA) cycling and de novo lipogenesis. The “healthy adipocytes” could be induced in WAT by various factors that need to be better characterized. “Healthy adipocytes” provide beneficial local and systemic effects that could contribute to “metabolically healthy” obesity. The role of the metabolic features of “healthy adipocytes” in the systemic effects of WAT needs to be better characterized.

The aim of this PhD project is to characterize adipose tissue metabolism in rodent models in stimulated and inactivated state (such as during cold exposure, in dietary-obese mice). In order to assess rate of the above mentioned metabolic processes, stable isotope labeling of newly synthetized lipids and metabolites will be used. Involvement of particular regulatory metabolic pathways will be evaluated using adeno-associated viruses-driven overexpression of certain proteins both in vivo and in cell culture model.

The basic scholarship will be eventually supplemented by salary covered by grants of the department.

Supervisor: Petra Janovska, Ph.D.; advisor: Jan Kopecky, MD, PhD

Candidate’s profile (requirements)

We are seeking a motivated candidate with Master degree in biology, medicine, biochemistry, or similar (or those who are expected to obtain the degree this year). Experience with work in techniques of biochemistry and molecular biology and with rodent models and cell cultures will be considered as an advantage. 

Relevant publications:

Flachs P. - Adamcová K. - Zouhar, P. - Marques C. - Janovská P. - Viegas I. - Jones J. G. - Bardová K. - Svobodová M. - Hansíková J. - Kuda O. - Rossmeisl M. - Liisberg U. - Borkowska A. G. - Kristiansen K. - Madsen L. - Kopecký J. Induction of lipogenesis in white fat during cold exposure in mice: link to lean phenotype. International Journal of Obesity. 2017,  41, 3, p. 372-380.

Rohm M. - Schäfer M. - Laurent V. - Üstünel B. E. - Niopek K. - Algire C. - Hautzinger O. - Sijmonsma T. P. - Zota A. - Medrikova D. - Pellegata N. S. - Ryden M. - Kulyte A. - Dahlman I. - Arner P. - Petrovic N. - Cannon B. - Amri E. Z. - Kemp B. E. - Steinberg G. R. - Janovská P. - Kopecký J. - Wolfrun Ch. - Blüher M. - Diaz M. B. - Herzig S. An AMP-activated protein kinase–stabilizing peptide ameliorates adipose tissue wasting in cancer cachexia in mice. Nature Medicine. 2016,  22, 10, p. 1120-1130.

Kuda, Ondrej -  Rossmeisl, Martin -  Kopecky, Jan. Omega-3 fatty acids and adipose tissue biology. Molecular Aspects of Medicine. 2018, 64, 147 – 160.