Our research focuses on the cellular and molecular mechanisms of desensitization of hormone response mediated by the activation of G protein coupled receptors (GPCR). G protein coupled receptors are plasma membrane integral proteins which serve as transducers of extracellular signals across the plasma membrane bilayer to the cell interior. GPCR play a key role in the regulation of many physiological processes and functions. Moreover, GPCRs represent one of the most important groups of targets for therapeutics.
The main areas of the research are:
Cell (plasma) membrane (PM) serves as a protective barrier, but also plays a role in the cellular communication. The integrity of hydrophobic lipid environment of PM is an important factor affecting function of membrane proteins.
MoreAddiction to opioid drugs (opioids) represents one of the most severe forms of drug abuse. Opium is one of the oldest known drugs and is used for its medicinal and euphoric effects for thousands of years. Opioid dependence develops as a consequence of changes in the central nervous system (CNS) induced by prolonged exposure to opioid drugs, e.g., morphine, codein or heroin.
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Activity of opioid receptor (OR) is among others modulates by monovalent cations especially by sodium (Na+) and lithium (Li+) which atomic radius is very similar to those of sodium. Lithium is used in psychiatry as a mood stabilizer (see review article Vošahlíková and Svoboda, 2016). Monovalent ions modulate GPCR signalling in not fully understood mechanism.
MoreUjčíková, Hana - Hejnová, Lucie - Eckhardt, Adam - Roubalová, Lenka - Novotný, Jiří - Svoboda, Petr . Impact of three-month morphine withdrawal on rat brain cortex, hippocampus, striatum and cerebellum: proteomic and phosphoproteomic studies. Neurochemistry International. 2021; 144(Mar); 104975 . IF = 3.881 [ASEP] [ doi ]
Brejchová, Jana - Holáň, Vladimír - Svoboda, Petr . Expression of Opioid Receptors in Cells of the Immune System. International Journal of Molecular Sciences. 2021; 22(1); 315 . IF = 4.556 [ASEP] [ doi ]
Vošahlíková; Miroslava - Roubalová; Lenka - Cechová; Kristína - Kaufman; Jonáš - Musil; Stanislav - Mikšík; Ivan - Alda; M. - Svoboda; Petr . Na+/K+-ATPase and lipid peroxidation in forebrain cortex and hippocampus of sleep-deprived rats treated with therapeutic lithium concentration for different periods of time . Progress in Neuro-Psychopharmacology & Biological Psychiatry. 2020; 102(Aug 30)); 109953 . IF = 4.361 [ASEP] [ doi ]
Ujčíková; Hana - Cechová; Kristína - Roubalová; Lenka - Brejchová; Jana - Kaufman; Jonáš - Holáň; Vladimír - Svoboda; Petr . The high-resolution proteomic analysis of protein composition of rat spleen lymphocytes stimulated by Concanavalin A; a comparison with morphine-treated cells . Journal of Neuroimmunology. 2020; 341(Apr 15)); 577191 . IF = 3.125 [ASEP] [ doi ]
Ujčíková; Hana - Cechová; Kristína - Jágr; Michal - Roubalová; Lenka - Vošahlíková; Miroslava - Svoboda; Petr . Proteomic analysis of protein composition of rat hippocampus exposed to morphine for 10 days; comparison with animals after 20 days of morphine withdrawal . PLoS ONE. 2020; 15(4)); e0231721 . IF = 2.740 [ASEP] [ doi ]
Melcrová; Adéla - Pokorná; Šárka - Vošahlíková; Miroslava - Sýkora; Jan - Svoboda; Petr - Hof; Martin - Cwiklik; Lukasz - Jurkiewicz; Piotr . Concurrent Compression of Phospholipid Membranes by Calcium and Cholesterol . Langmuir. 2019; 35(35); 11358-11368 . IF = 3.683 [ASEP] [ doi ]
Vošahlíková, Miroslava - Roubalová, Lenka - Ujčíková, Hana - Hloušková, Martina - Musil, Stanislav - Alda, M. - Svoboda, Petr . Na+/K+-ATPase level and products of lipid peroxidation in live cells treated with therapeutic lithium for different periods in time (1, 7, and 28 days); studies of Jurkat and HEK293 cells . Naunyn-Schmiedeberg's Archives of Pharmacology. 2019, 392(7), 785-799 . IF = 2.058 [ASEP] [ doi ]
Čočková; Z. - Ujčíková; Hana - Telenský; P. - Novotný; J. Protein profiling of SH-SY5Y neuroblastoma cells: The effect of rhein . Journal of Biosciences. 2019; 44(4)); UNSP88 . IF = 1.823 [ASEP] [ doi ]
Janáček; Jiří - Brejchová; Jana - Svoboda; Petr . Determination of delta-opioid receptor molecules mobility in living cells plasma membrane by novel method of FRAP analysis . Biochimica Et Biophysica Acta-Biomembranes. 2019; 1861(7); 1364-1354 . IF = 3.790 [ASEP] [ doi ]
Vošahlíková, Miroslava - Ujčíková, Hana - Hloušková, Martina - Musil, Stanislav - Roubalová, Lenka - Alda, M. - Svoboda, Petr . Induction of oxidative stress by long-term treatment of live HEK293 cells with therapeutic concentration of lithium is associated with down-regulation of delta-opioid receptor amount and function . Biochemical Pharmacology 2018, roč. 154, Aug, p. 452-463 . IF = 4.825 [ASEP] [ doi ]
Holáň, Vladimír - Cechová, Kristína - Zajícová, Alena - Kössl, Jan - Heřmánková, Barbora - Boháčová, Pavla - Hájková, Michaela - Krulová, Magdaléna - Svoboda, Petr - Javorková, Eliška . The Impact of Morphine on the Characteristics and Function Properties of Human Mesenchymal Stem Cells . Stem Cell Reviews and Reports 2018, 14(6), 801-811 . IF = 4.697 [ASEP] [ doi ]
Cechová, Kristína - Hloušková, Martina - Javorková, Eliška - Roubalová, Lenka - Ujčíková, Hana - Holáň, Vladimír - Svoboda, Petr . Up-regulation of mu-, delta- and kappa-opioid receptors in concanavalin A-stimulated rat spleen lymphocytes . Journal of Neuroimmunology 2018, 321(Aug 15), 12-23 . IF = 2.832 [ASEP] [ doi ]
Vošahlíková, Miroslava - Ujčíková, Hana - Chernyavskiy, Oleksandr - Brejchová, Jana - Roubalová, Lenka - Alda, M. - Svoboda, Petr . Effect of therapeutic concentration of lithium on live HEK293 cells; increase of Na+/K+-ATPase, change of overall protein composition and alteration of surface layer of plasma membrane . Biochimica et Biophysica Acta. General Subjects. 2017, roč. 1861, 5, p. 1099-1112. IF = 3.679 [ASEP] [ doi ]
Ujčíková, Hana - Hloušková, Martina - Cechová, Kristina - Stolařová, Kateřina - Roubalová, Lenka - Svoboda, Petr . Determination of mu-, delta- and kappa-opioid receptors in forebrain cortex of rats exposed to morphine for 10 days: Comparison with animals after 20 days of morphine withdrawal . PLoS ONE. 2017, roč. 12, 10, článku e0186797. IF = 2.766 [ASEP] [ doi ]
Vošahlíková, Miroslava - Svoboda, Petr. Lithium - therapeutic tool endowed with multiple beneficiary effects caused by multiple mechanisms. Acta Neurobiologiae Experimentalis. 2016, roč.76, 1, p. 1-19. ISSN 0065-1400. IF = 1.708 [ASEP]
Ujčíková, Hana - Vošahlíková, Miroslava - Roubalová, Lenka - Svoboda, Petr. Proteomic analysis of protein composition of rat forebrain cortex exposed to morphine for 10 days; comparison with animals after 20 days of morphine withdrawal. Journal of Proteomics. 2016, roč. 145, p. 11-23. ISSN 1874-3919. IF =3.867 [ASEP] [ doi ]
Brejchová, Jana - Vošahlíková, Miroslava - Roubalová, Lenka - Parenti, Marco - Mauri, Mario - Chernyavskiy, Oleksandr - Svoboda, Petr. Plasma membrane cholesterol level and agonist-induced internalization of δ-opioid receptors; colocalization study with intracellular membrane markers of Rab family. Journal of Bioenergetics and Biomembranes. 2016, roč. 48, p.375-396. ISSN 0145-479X IF = 2.080 [ doi ]
Kohutiar, Matej - Ivica, Josko - Vytášek, Richard - Skoumalová Alice - Illner Jan - Šantorová Pavlína - Wilhelm, Jiří. Comparison of the effects of tert-butyl hydroperoxide and peroxynitrite on the oxidative damage to isolated beef heart mitochondria. Physiological Research. 2016, roč. 65,4, p.617-626. ISSN 0862-8408 IF =1.618
Roubalová, Lenka - Vošahlíková, Miroslava - Brejchová, Jana - Sýkora, Jan - Rudajev, Vladimír - Svoboda, Petr. High Efficacy but Low Potency of delta-Opioid Receptor-G Protein Coupling in Brij-58-Treated, Low-Density Plasma Membrane Fragments. PLoS ONE. 2015, roč. 10, 8, e0135664. ISSN 1932-6203. IF =3.057 [ASEP] [ doi ]
Wilhelm, Jiří - Ivica, Josko - Veselská, Zdeňka - Uhlík, Jiří - Vajner, Luděk. Changes in the composition of fatty acids and lipofuscin-like pigments during development of rat heart. Physiological Research. 2015, roč. 64, 5, p. 643-651. ISSN 0862-8408. IF =1.618 [ASEP]
Brejchová, Jana - Sýkora, Jan - Ostašov, Pavel - Merta, Ladislav - Roubalová, Lenka - Janáček, Jiří - Hof, Martin - Svoboda, Petr. TRH-receptor mobility and function in intact and cholesterol-depleted plasma membrane of HEK293 cells stably expressing TRH-R-eGFP . Biochimica Et Biophysica Acta-Biomembranes. 2015, roč. 1848, 3, p. 781-796 . ISSN 0005-2736. IF =3.687 [ASEP] [ doi ]
