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Hematological testing of blood / plasma / serum from test animals

Technical details of hematological testing 

Animal species: mouse, rat, dog, cat, horse, ferret

Measured parameters: WBC, RBC, HGB, HCT, MCV, MCH, MCHC, RDW, PLT, MPV, LYM, MON, GRA, EOS
Animal species: rabbit, pig, sheep, cattle

Measured parameters: WBC, RBC, HGB, HCT, MCV, MCH, MCHC, PLT

Minimum sample volume: 50 μl EDTA blood
Blood measurement should be done within 4 hours after blood collection

Technical specifications
Method: RBC, WBC and PLTS- electrical impedance, hemoglobin - spectral photometry


scil Vet abc Plus+: Analytical instrument to determine hematological parameters from whole blood


Coagulation parameters testing from blood plasma of test animals

Technical details

 Animal species: rat

Measured parameters: APTT, PT

Minimum sample volume: 60 μl blood citrate plasma

Rayto RT-2204 C Analytical instrument to determine coagulation parameters from blood plasma


Citrate plasma preparation

Venous blood should be carefully collected into a tube treated by sodium citrate solution. Attention should be paid to maintain of ratio of 9 parts blood to 1 part citrate. Tubes with blood should be mixed well and centrifuged (15 minutes, 2500 g, 20 °C) as soon as possible after blood collection. Plasma samples should be maintained at room temperature and should be tested within 4 hours by PT test and within 8 hours by APTT test.

Untested residue of citrate plasma should be processed to platelet-free citrate plasma by second centrifugation (30 minutes, 3000 g, 20 °C). Separated platelet-free plasma should be immediately frozen at -80 °C for further possible testing.

Frozen platelet-free citrate plasma should be thawed at 37 °C for 15 minutes and then should be mixed well and centrifuged (5 minutes, 500 RPM, 20 °C). The plasma should be tested immediately after centrifugation.