Cutting-edge science for health

Laboratory of Biomathematics

Laboratory of Biomathematics

The Laboratory of Biomathematics consists of two research groups: (1) Bioimaging and Image Analysis, and (2) Biochemistry of Membrane Receptors. Operating mainly at in situ and in vitro levels, respectively, the two groups complement each other when studying phenomena confined to the cell membrane.

1.      Bioimaging and Image Analysis

The group conducts research into the 3D microanatomical aspects of physiological phenomena at mesoscopic, microscopic and ultrastructural level. It is engaged in a range of collaborative projects across the campus and beyond. If interested please contact us (


  • Reconstruction and registration of images acquired by multimodal preclinical imaging methods
  • The conduction system of developing murine heart


  • Development of 3D image analysis and stereological methods including software (employed, e.g., in a volumetric analysis of Langerhans islets from human donors)
  • Microscopic optical-contrasting (label-free) strategies to visualize living cells and tissues

OTHER PROJECTS (selection only)

  • Effects of ionizing radiation on blood capillaries network
  • Effects of elevated CO2 on the photosynthetic apparatus’ performance and  ultrastructure
  • Mechanisms governing the formation of extracellular matrix proteins (collagen and elastin)


  • Laser scanning confocal microscopy
  • Two-photon excitation microscopy
  • Second harmonic generation (SHG) microscopy
  •  ‘White-light‘ laser and hybrid detectors (on Leica TCS SP8 WLL MP microscope)
  • FRAP recording, FRET analysis, FLIM/PLIM recording including λ2 scans 
  • Optical projection tomography (OPT)
  • Selective plane illumination (light sheet) microscopy (SPIM)
  • Phase-, modulation- and interference contrast microscopy


  • Software for 3D image analysis, stereology, spatial statistics and deconvolution (Hyughens)
  • Optical/chemical clearing protocols for OPT and SPIM


The group is part of the Czech BioImaging infrastructure [1] supporting open access to most of its microscopes (listed above), and implicitly also to its expertise. The portfolio includes, among other imaging equipment located at other departments, a Nipkow spinning-disk (“Carv II“) confocal microscope [2].




2.      Biochemistry of Membrane Receptors

Our research focuses on the cellular and molecular mechanisms of desensitization of hormone response mediated by the activation of G protein coupled receptors (GPCR). G protein coupled receptors are plasma membrane integral proteins which serve as transducers of extracellular signals across the plasma membrane bilayer to the cell interior. GPCR play a key role in the regulation of many physiological processes and functions. Moreover, GPCRs represent one of the most important groups of targets for therapeutics.

The main areas of the research are:

  • The role of cell membrane and membrane domains
  • Opioid receptors and drug addiction
  • The effect of monovalent ions on δ-opioid receptors – analysis of lithium effect in living cells and isolated cell membranes
  • GABA-B - signalling cascades

Detail informations here.




Vošahlíková; Miroslava - Roubalová; Lenka - Cechová; Kristína - Kaufman; Jonáš - Musil; Stanislav - Mikšík; Ivan - Alda; M. - Svoboda; Petr . Na+/K+-ATPase and lipid peroxidation in forebrain cortex and hippocampus of sleep-deprived rats treated with therapeutic lithium concentration for different periods of time . Progress in Neuro-Psychopharmacology & Biological Psychiatry. 2020; 102(Aug 30)); 109953 . IF = 4.361 [ASEP] [ doi ]
Ujčíková; Hana - Cechová; Kristína - Roubalová; Lenka - Brejchová; Jana - Kaufman; Jonáš - Holáň; Vladimír - Svoboda; Petr . The high-resolution proteomic analysis of protein composition of rat spleen lymphocytes stimulated by Concanavalin A; a comparison with morphine-treated cells . Journal of Neuroimmunology. 2020; 341(Apr 15)); 577191 . IF = 3.125 [ASEP] [ doi ]
Ujčíková; Hana - Cechová; Kristína - Jágr; Michal - Roubalová; Lenka - Vošahlíková; Miroslava - Svoboda; Petr . Proteomic analysis of protein composition of rat hippocampus exposed to morphine for 10 days; comparison with animals after 20 days of morphine withdrawal . PLoS ONE. 2020; 15(4)); e0231721 . IF = 2.740 [ASEP] [ doi ]
Pajorová; Julia - Skogberg; A. - Hadraba; Daniel - Brož; Antonín - Trávníčková; Martina - Zikmundová; Markéta - Honkanen; M. - Hannula; M. - Lahtinen; P. - Tomková; M. - Bačáková; Lucie - Kallio; P. Cellulose Mesh with Charged Nanocellulose Coatings as a Promising Carrier of Skin and Stem Cells for Regenerative Applications . Biomacromolecules. 2020; 21(12); 4857-4870 . IF = 6.092 [ASEP] [ doi ]
Musílková; Jana - Filová; Elena - Pala; J. - Matějka; Roman - Hadraba; Daniel - Vondrášek; David - Kaplan; Ondřej - Riedel; Tomáš - Brynda; Eduard - Kučerová; Johanka - Koňařík; M. - Lopot; F. - Pirk; J. - Bačáková; Lucie . Human decellularized and crosslinked pericardium coated with bioactive molecular assemblies . Biomedical Materials. 2020; 15(1)); 015008 . IF = 3.174 [ASEP] [ doi ]
Havelka; Daniel - Chafai; Djamel Eddine - Krivosudský; Ondrej - Klebanovych; Anastasiya - Vostárek; František - Kubínová; Lucie - Dráber; Pavel - Cifra; Michal . Nanosecond Pulsed Electric Field Lab-on-Chip Integrated in Super-Resolution Microscope for Cytoskeleton Imaging . Advanced Materials Technologies. 2020; 5(3)); 1900669 . IF = 5.969 [ASEP] [ doi ]
Filová; Elena - Staňková; Ľubica - Eckhardt; Adam - Svobodová; Jana - Musílková; Jana - Pala; J. - Hadraba; Daniel - Brynda; Eduard - Koňařík; M. - Pirk; J. - Bačáková; Lucie . Modification of Human Pericardium by Chemical Crosslinking . Physiological Research. 2020; 69(1); 49-59 . IF = 1.655 [ASEP] [ doi ]
Vošahlíková, Miroslava - Roubalová, Lenka - Ujčíková, Hana - Hloušková, Martina - Musil, Stanislav - Alda, M. - Svoboda, Petr . Na+/K+-ATPase level and products of lipid peroxidation in live cells treated with therapeutic lithium for different periods in time (1, 7, and 28 days); studies of Jurkat and HEK293 cells . Naunyn-Schmiedeberg's Archives of Pharmacology 2019, 392(7), 785-799 . IF = 2.050 [ASEP] [ doi ]
Umek; N. - Horvat; S. - Cvetko; E. - Kreft; M. - Janáček; Jiří - Kubínová; Lucie - Pintaric; T.S. - Eržen; I. 3D analysis of capillary network in skeletal muscle of obese insulin-resistant mice . Histochemistry and Cell Biology. 2019; 152(5); 323-331 . IF = 3.418 [ASEP] [ doi ]
Svoboda, Jan - Litvinec, Andrej - Kala, David - Pošusta, Antonín - Vávrová, Lenka - Jiruška, Přemysl - Otáhal, Jakub . Strain Differences in Intraluminal Thread Model of Middle Cerebral Artery Occlusion in Rats . Physiological Research 2019, 68(1), 37-48 . IF = 1.655 [ASEP] [ doi ]

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RNDr. Jiří Janáček, PhD.
Head of the Laboratory
Ing. Martin Čapek, PhD.
Senior Researcher (part-time)
RNDr. Barbora Radochová, PhD.
Senior Researcher
Ing. Mgr. Daniel Hadraba, Ph.D.
Postdoctoral Researcher
MUDr. Petr Karen, CSc.
Senior Researcher

Mgr. David Kala

PhD Student


Michaela Vodičková

PhD Student

Mgr. David Vondrášek


Mgr. Zuzana Kubínová


Ing. Jitka Čočková
Project Manager

Bc. Jonáš Kaufman

Research Assistant


Mgr. Diana Siswartonová

Research Assistant


Ivana Švandová

Research Assistant


Petr Zvolánek

Research Assistant



Biochemistry of Membrane Receptors

Doc. RNDr. Petr Svoboda, DrSc.
Senior researcher
RNDr. Lenka Roubalová, PhD.
Senior researcher
RNDr. Hana Ujčíková, PhD.
Senior researcher
Ing. Miroslava Vošahlíková, PhD.
Senior researcher
Mgr. Jana Brejchová, PhD.
Senior researcher
  Mgr. Kristina Cechová
PhD student