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INSTITUTE OF PHYSIOLOGY CAS

Cutting-edge science for health

Laboratory of Biomathematics

Laboratory of Biomathematics

The Laboratory of Biomathematics consists of two research groups: (1) Bioimaging and Image Analysis, and (2) Biochemistry of Membrane Receptors. Operating mainly at in situ and in vitro levels, respectively, the two groups complement each other when studying phenomena confined to the cell membrane.

1.      Bioimaging and Image Analysis

The group conducts research into the 3D microanatomical aspects of physiological phenomena at mesoscopic, microscopic and ultrastructural level. It is engaged in a range of collaborative projects across the campus and beyond. If interested please contact us (jiri.janacek@fgu.cas.cz).

CURRENTLY FUNDED PROJECTS

  • Reconstruction and registration of images acquired by multimodal preclinical imaging methods
  • The conduction system of developing murine heart

LONG-TERM PROJECTS

  • Development of 3D image analysis and stereological methods including software (employed, e.g., in a volumetric analysis of Langerhans islets from human donors)
  • Microscopic optical-contrasting (label-free) strategies to visualize living cells and tissues

OTHER PROJECTS (selection only)

  • Effects of ionizing radiation on blood capillaries network
  • Effects of elevated CO2 on the photosynthetic apparatus’ performance and  ultrastructure
  • Mechanisms governing the formation of extracellular matrix proteins (collagen and elastin)

IN-HOUSE IMAGING MODALITIES

  • Laser scanning confocal microscopy
  • Two-photon excitation microscopy
  • Second harmonic generation (SHG) microscopy
  •  ‘White-light‘ laser and hybrid detectors (on Leica TCS SP8 WLL MP microscope)
  • FRAP recording, FRET analysis, FLIM/PLIM recording including λ2 scans 
  • Optical projection tomography (OPT)
  • Selective plane illumination (light sheet) microscopy (SPIM)
  • Phase-, modulation- and interference contrast microscopy

AVAILABLE SOFTWARE AND PROTOCOLS

  • Software for 3D image analysis, stereology, spatial statistics and deconvolution (Hyughens)
  • Optical/chemical clearing protocols for OPT and SPIM

OPEN ACCESS TO EQUIPMENT

The group is part of the Czech BioImaging infrastructure [1] supporting open access to most of its microscopes (listed above), and implicitly also to its expertise. The portfolio includes, among other imaging equipment located at other departments, a Nipkow spinning-disk (“Carv II“) confocal microscope [2].

[1]      http://www.czech-bioimaging.cz/

[2]      http://www.fgu.cas.cz/en/articles/529-czech-bioimaging-2016-2019

 

2.      Biochemistry of Membrane Receptors

Our research focuses on the cellular and molecular mechanisms of desensitization of hormone response mediated by the activation of G protein coupled receptors (GPCR). G protein coupled receptors are plasma membrane integral proteins which serve as transducers of extracellular signals across the plasma membrane bilayer to the cell interior. GPCR play a key role in the regulation of many physiological processes and functions. Moreover, GPCRs represent one of the most important groups of targets for therapeutics.

The main areas of the research are:

  • The role of cell membrane and membrane domains
  • Opioid receptors and drug addiction
  • The effect of monovalent ions on δ-opioid receptors – analysis of lithium effect in living cells and isolated cell membranes
  • GABA-B - signalling cascades

Detail informations here.

Projects

Achievements

Publications

Vošahlíková; Miroslava - Roubalová; Lenka - Cechová; Kristína - Kaufman; Jonáš - Musil; Stanislav - Mikšík; Ivan - Alda; M. - Svoboda; Petr . Na+/K+-ATPase and lipid peroxidation in forebrain cortex and hippocampus of sleep-deprived rats treated with therapeutic lithium concentration for different periods of time . Progress in Neuro-Psychopharmacology & Biological Psychiatry. 2020; 102(Aug 30)); 109953 . IF = 4.361 [ASEP] [ doi ]
Ujčíková; Hana - Cechová; Kristína - Roubalová; Lenka - Brejchová; Jana - Kaufman; Jonáš - Holáň; Vladimír - Svoboda; Petr . The high-resolution proteomic analysis of protein composition of rat spleen lymphocytes stimulated by Concanavalin A; a comparison with morphine-treated cells . Journal of Neuroimmunology. 2020; 341(Apr 15)); 577191 . IF = 3.125 [ASEP] [ doi ]
Ujčíková; Hana - Cechová; Kristína - Jágr; Michal - Roubalová; Lenka - Vošahlíková; Miroslava - Svoboda; Petr . Proteomic analysis of protein composition of rat hippocampus exposed to morphine for 10 days; comparison with animals after 20 days of morphine withdrawal . PLoS ONE. 2020; 15(4)); e0231721 . IF = 2.740 [ASEP] [ doi ]
Pajorová; Julia - Skogberg; A. - Hadraba; Daniel - Brož; Antonín - Trávníčková; Martina - Zikmundová; Markéta - Honkanen; M. - Hannula; M. - Lahtinen; P. - Tomková; M. - Bačáková; Lucie - Kallio; P. Cellulose Mesh with Charged Nanocellulose Coatings as a Promising Carrier of Skin and Stem Cells for Regenerative Applications . Biomacromolecules. 2020; 21(12); 4857-4870 . IF = 6.092 [ASEP] [ doi ]
Musílková; Jana - Filová; Elena - Pala; J. - Matějka; Roman - Hadraba; Daniel - Vondrášek; David - Kaplan; Ondřej - Riedel; Tomáš - Brynda; Eduard - Kučerová; Johanka - Koňařík; M. - Lopot; F. - Pirk; J. - Bačáková; Lucie . Human decellularized and crosslinked pericardium coated with bioactive molecular assemblies . Biomedical Materials. 2020; 15(1)); 015008 . IF = 3.174 [ASEP] [ doi ]
Havelka; Daniel - Chafai; Djamel Eddine - Krivosudský; Ondrej - Klebanovych; Anastasiya - Vostárek; František - Kubínová; Lucie - Dráber; Pavel - Cifra; Michal . Nanosecond Pulsed Electric Field Lab-on-Chip Integrated in Super-Resolution Microscope for Cytoskeleton Imaging . Advanced Materials Technologies. 2020; 5(3)); 1900669 . IF = 5.969 [ASEP] [ doi ]
Filová; Elena - Staňková; Ľubica - Eckhardt; Adam - Svobodová; Jana - Musílková; Jana - Pala; J. - Hadraba; Daniel - Brynda; Eduard - Koňařík; M. - Pirk; J. - Bačáková; Lucie . Modification of Human Pericardium by Chemical Crosslinking . Physiological Research. 2020; 69(1); 49-59 . IF = 1.655 [ASEP] [ doi ]
Vošahlíková, Miroslava - Roubalová, Lenka - Ujčíková, Hana - Hloušková, Martina - Musil, Stanislav - Alda, M. - Svoboda, Petr . Na+/K+-ATPase level and products of lipid peroxidation in live cells treated with therapeutic lithium for different periods in time (1, 7, and 28 days); studies of Jurkat and HEK293 cells . Naunyn-Schmiedeberg's Archives of Pharmacology 2019, 392(7), 785-799 . IF = 2.050 [ASEP] [ doi ]
Umek; N. - Horvat; S. - Cvetko; E. - Kreft; M. - Janáček; Jiří - Kubínová; Lucie - Pintaric; T.S. - Eržen; I. 3D analysis of capillary network in skeletal muscle of obese insulin-resistant mice . Histochemistry and Cell Biology. 2019; 152(5); 323-331 . IF = 3.418 [ASEP] [ doi ]
Svoboda, Jan - Litvinec, Andrej - Kala, David - Pošusta, Antonín - Vávrová, Lenka - Jiruška, Přemysl - Otáhal, Jakub . Strain Differences in Intraluminal Thread Model of Middle Cerebral Artery Occlusion in Rats . Physiological Research 2019, 68(1), 37-48 . IF = 1.655 [ASEP] [ doi ]
Olejníčková, Veronika - Šaňková, Barbora - Sedmera, David - Janáček, Jiří . Trabecular Architecture Determines Impulse Propagation Through the Early Embryonic Mouse Heart . Frontiers in physiology 2019, 9(Jan 8)), 1876 . IF = 3.367 [ASEP] [ doi ]
Melcrová; Adéla - Pokorná; Šárka - Vošahlíková; Miroslava - Sýkora; Jan - Svoboda; Petr - Hof; Martin - Cwiklik; Lukasz - Jurkiewicz; Piotr . Concurrent Compression of Phospholipid Membranes by Calcium and Cholesterol . Langmuir. 2019; 35(35); 11358-11368 . IF = 3.557 [ASEP] [ doi ]
Kubínová, Z. - Glanc, N. - Radochová, Barbora - Lhotáková, Z. - Janáček, Jiří - Kubínová, Lucie - Albrechtová, J. Unbiased estimation of Norway spruce (Picea abies L. Karst.) chloroplast structure: Heterogeneity within needle mesophyll under different irradiance and [CO 2 ] . Image Analysis and Stereology 2019, 38(1), 83-94 . IF = 0.550 [ASEP] [ doi ]
Krok-Borkowicz, M. - Filová, Elena - Chlupáč, Jaroslav - Klepetář, Jan - Bačáková, Lucie - Pamula, E. Influence of pore size and hydroxyapatite deposition in poly(L-lactide-co-glycolide) scaffolds on osteoblast-like cells cultured in static and dynamic conditions . Materials Letters 2019, 241(Apr 15), 1-5 . IF = 3.204 [ASEP] [ doi ]
Janáček; Jiří - Jirák; D. Variance of the isotropic uniform systematic sampling . Image Analysis and Stereology. 2019; 38(3); 261-267 . IF = 0.550 [ASEP] [ doi ]
Janáček; Jiří - Jirák; D. Volume tensor estimation using a virtual line grid: study of a developing pheasant brain . Image Analysis and Stereology. 2019; 38(3); 255-260 . IF = 0.550 [ASEP] [ doi ]
Janáček; Jiří - Brejchová; Jana - Svoboda; Petr . Determination of delta-opioid receptor molecules mobility in living cells plasma membrane by novel method of FRAP analysis . Biochimica Et Biophysica Acta-Biomembranes. 2019; 1861(7); 1364-1354 . IF = 3.411 [ASEP] [ doi ]
Chafai; Djamel Eddine - Sulimenko; Vadym - Havelka; Daniel - Kubínová; Lucie - Dráber; Pavel - Cifra; Michal . Reversible and Irreversible Modulation of Tubulin Self-Assembly by Intense Nanosecond Pulsed Electric Fields . Advanced Materials. 2019; 31(39)); e1903636 . IF = 27.398 [ASEP] [ doi ]
Eckhardt, Adam - Novotný, T. - Doubková, Martina - Hronková, Lucia - Vajner, L. - Pataridis, Statis - Hadraba, Daniel - Kulhavá, Lucie - Plencner, Martin - Knitlová, Jarmila - Lišková, Jana - Uhlík, J. - Žaloudíková, M. - Vondrášek, David - Mikšík, Ivan - Ošťádal, M. Novel contribution to clubfoot pathogenesis: The possible role of extracellular matrix proteins . Journal of Orthopaedic Research 2019, 37(3), 769-778 . IF = 2.728 [ASEP] [ doi ]
Čočková; Z. - Ujčíková; Hana - Telenský; P. - Novotný; J. Protein profiling of SH-SY5Y neuroblastoma cells: The effect of rhein . Journal of Biosciences. 2019; 44(4)); UNSP88 . IF = 1.645 [ASEP] [ doi ]
Beyrand; V. - Voeten; D. F. A. E. - Bureš; S. - Fernandez; V. - Janáček; Jiří - Jirák; D. - Rauhut; O. - Tafforeau; P. Multiphase progenetic development shaped the brain of flying archosaurs . Scientific Reports. 2019; 9(Jul 25)); 10807 . IF = 3.998 [ASEP] [ doi ]
Bačáková; Markéta - Pajorová; Julia - Brož; Antonín - Hadraba; Daniel - Lopot; F. - Zavaďáková; A. - Vištejnová; L. - Beňo; M. - Kostič; I. - Jenčová; V. - Bačáková; Lucie . A two-layer skin construct consisting of a collagen hydrogel reinforced by a fibrin-coated polylactide nanofibrous membrane . International Journal of Nanomedicine. 2019; 14(2019); 5033-5050 . IF = 5.115 [ASEP] [ doi ]
Vošahlíková, Miroslava - Ujčíková, Hana - Hloušková, Martina - Musil, Stanislav - Roubalová, Lenka - Alda, M. - Svoboda, Petr . Induction of oxidative stress by long-term treatment of live HEK293 cells with therapeutic concentration of lithium is associated with down-regulation of delta-opioid receptor amount and function . Biochemical Pharmacology 2018, roč. 154, Aug, p. 452-463 . IF = 4.825 [ASEP] [ doi ]
Vetrík, Miroslav - Pařízek, Martin - Hadraba, Daniel - Kukačková, Olivia - Brus, Jiří - Hlídková, Helena - Kománková, Lucie - Hodan, Jiří - Sedláček, Ondřej - Šlouf, Miroslav - Bačáková, Lucie - Hrubý, Martin . Porous heat-treated polyacrylonitrile scaffolds for bone tissue engineering . ACS Applied Materials and Interfaces 2018, roč. 10, 10, p. 8496-8506 . IF = 8.456 [ASEP] [ doi ]
Prats-Galino, A. - Čapek, Martin - Reina, M. A. - Cvetko, E. - Radochová, Barbora - Tubbs, R.S. - Damjanovska, M. - Pintaric, T.S. 3D reconstruction of peripheral nerves from optical projection tomography images: A method for studying fascicular interconnections and intraneural plexuses . Clinical Anatomy 2018, roč. 31, 3, p. 424-431 . IF = 1.813 [ASEP] [ doi ]
Pajorová, Julie - Bačáková, Markéta - Musílková, Jana - Brož, Antonín - Hadraba, Daniel - Lopot, F. - Bačáková, Lucie . Morphology of a fibrin nanocoating influences dermal fibroblast behavior . International Journal of Nanomedicine 2018, roč. 13, June, p. 3367-3380 . IF = 4.471 [ASEP] [ doi ]
Meznaric, M. - Eržen, I. - Karen, Petr - Cvetko, E. Effect of ageing on the myosin heavy chain composition of the human sternocleidomastoid muscle . Annals of Anatomy-Anatomischer Anzeiger 2018, roč. 216, March, p. 95-99 . IF = 2.241 [ASEP] [ doi ]
Kubínová, Z. - Janáček, Jiří - Lhotáková, Z. - Šprtová, Miroslava - Kubínová, Lucie - Albrechtová, Jana . Norway spruce needle size and cross section shape variability induced by irradiance on a macro- and microscale and CO2 concentration . Trees: structure and function 2018, roč. 32, 1, p. 231-244 . IF = 1.799 [ASEP] [ doi ]
Kohutiar, Matej - Eckhardt, Adam - Mikšík, Ivan - Šantorová, J. - Wilhelm, Jiří . Proteomic analysis of peroxynitrite-induced protein nitration in isolated beef heart mitochondria . Physiological Research 2018, roč. 67, 2, p. 239-250 . IF = 1.701 [ASEP]
Holáň, Vladimír - Cechová, Kristína - Zajícová, Alena - Kössl, Jan - Heřmánková, Barbora - Boháčová, Pavla - Hájková, Michaela - Krulová, Magdaléna - Svoboda, Petr - Javorková, Eliška . The Impact of Morphine on the Characteristics and Function Properties of Human Mesenchymal Stem Cells . Stem Cell Reviews and Reports 2018, 14(6), 801-811 . IF = 4.697 [ASEP] [ doi ]
Eržen, I. - Janáček, Jiří - Kreft, M. - Kubínová, Lucie - Cvetko, E. Capillary Network Morphometry of Pig Soleus Muscle Significantly Changes in 24 Hours After Death . Journal of Histochemistry and Cytochemistry 2018, roč. 66, 1, p. 23-31 . IF = 2.370 [ASEP] [ doi ]
Dvořák, J. - Švihlík, J. - Kybic, J. - Radochová, Barbora - Janáček, Jiří - Kukal, J. - Borovec, J. - Habart, D. Volume estimation from single images: an application to pancreatic islets. Image Analysis and Stereology 2018, 37(3), 191-204 . IF = 1.778 [ASEP] [ doi ]
Czerneková, Michaela - Jönsson, K. I. - Hajer, J. - Devetter, Miloslav . Evaluation of extraction methods for quantitative analysis of tardigrade populations in soil and leaf litter . Pedobiologia 2018, roč. 70, Sep, p. 1-5 . IF = 1.833 [ASEP] [ doi ]
Czerneková, Michaela - Janelt, K. - Student, S. - Jönsson, K. I. - Poprawa, I. A comparative ultrastructure study of storage cells in the eutardigrade Richtersius coronifer in the hydrated state and after desiccation and heating stress . PLoS ONE 2018, roč. 13, 8, článku e0201430 . IF = 2.776 [ASEP] [ doi ]
Cechová, Kristína - Hloušková, Martina - Javorková, Eliška - Roubalová, Lenka - Ujčíková, Hana - Holáň, Vladimír - Svoboda, Petr . Up-regulation of mu-, delta- and kappa-opioid receptors in concanavalin A-stimulated rat spleen lymphocytes . Journal of Neuroimmunology 2018, 321(Aug 15), 12-23 . IF = 2.832 [ASEP] [ doi ]
Boušová, Kristýna - Herman, P. - Večeř, J. - Bednárová, L. - Monincová, L. - Majer, P. - Vyklický ml., Ladislav - Vondrášek, J. - Teisinger, Jan . Shared CaM- and S100A1-binding epitopes in the distal TRPM4 N terminus . FEBS Journal 2018, roč. 285, 3, p. 599-613 . IF = 4.739 [ASEP] [ doi ]
Ziegler, A. - Neues, F. - Janáček, Jiří - Beckmann, F. - Epple, M. Mineral in skeletal elements of the terrestrial crustacean Porcellio scaber: SR mu CT of function related distribution and changes during the moult cycle . Arthropod Structure & Development. 2017, roč. 46, 1, p. 63-76 . IF = 1.696 [ASEP] [ doi ]
Vošahlíková, Miroslava - Ujčíková, Hana - Chernyavskiy, Oleksandr - Brejchová, Jana - Roubalová, Lenka - Alda, M. - Svoboda, Petr . Effect of therapeutic concentration of lithium on live HEK293 cells; increase of Na+/K+-ATPase, change of overall protein composition and alteration of surface layer of plasma membrane . Biochimica et Biophysica Acta. General Subjects. 2017, roč. 1861, 5, p. 1099-1112 . IF = 3.679 [ASEP] [ doi ]
Ujčíková, Hana - Hloušková, Martina - Cechová, Kristina - Stolařová, Kateřina - Roubalová, Lenka - Svoboda, Petr . Determination of mu-, delta- and kappa-opioid receptors in forebrain cortex of rats exposed to morphine for 10 days: Comparison with animals after 20 days of morphine withdrawal . PLoS ONE. 2017, roč. 12, 10, článku e0186797 . IF = 2.766 [ASEP] [ doi ]
Techniková, L. - Tunák, M. - Janáček, Jiří . New objective system of pilling evaluation for various types of fabrics . Textile Institute. Journal. 2017, roč. 108, 1, p. 123-131 . IF = 1.174 [ASEP] [ doi ]
Ošťádal, M. - Lišková, Jana - Hadraba, Daniel - Eckhardt, Adam . Possible Pathogenetic Mechanisms and New Therapeutic Approaches of Pes Equinovarus . Physiological Research. 2017, roč. 66, 3, p. 403-410 . IF = 1.324 [ASEP]
Mašková, P. - Radochová, Barbora - Lhotáková, Z. - Michálek, Jan - Lipavská, H. Nonstructural carbohydrate-balance response to long-term elevated CO2 exposure in European beech and Norway spruce mixed cultures: biochemical and ultrastructural responses . Canadian Journal of Forest Research. 2017, roč. 47, 11, p. 1498-1494 . IF = 1.887 [ASEP] [ doi ]
Lišková, Jana - Hadraba, Daniel - Filová, Elena - Koňařík, M. - Pirk, J. - Jelen, K. - Bačáková, Lucie . Valve interstitial cell culture: Production of mature type I collagen and precise detection . Microscopy Research and Technique. 2017, roč. 80, 8, p. 936-942 . IF = 1.087 [ASEP] [ doi ]
Kubínová, Lucie - Radochová, Barbora - Lhotáková, Z. - Kubínová, Z. - Albrechtová, J. Stereology, an unbiased methodological approach to study plant anatomy and cytology: Past, present and future . Image Analysis and Stereology.. 2017, roč. 36, 3, p. 187-205 . IF = 1.424 [ASEP] [ doi ]
Koshel, E. I. - Cheluskin, P. S. - Melnikov, A. S. - Serdobintsev, P. Y. - Stolbovaia, A. Y. - Saifitdinova, A. F. - Scheslavskiy, V. I. - Chernyavskiy, Oleksandr - Gaginskaya, E. R. - Koshevoy, I. O. - Tunik, S. P. Lipophilic phosphorescent gold(I) clusters as selective probes for visualization of lipid droplets by two-photon microscopy . Journal of Photochemistry and Photobiology A-Chemistry. 2017, roč. 332, Jan 1, p. 122-130 . IF = 2.891 [ASEP] [ doi ]
Jirák, D. - Janáček, Jiří . Volume of the crocodilian brain and endocast during ontogeny . PLoS ONE. 2017, roč. 12, 6, článku e0178491 . IF = 2.766 [ASEP] [ doi ]
Hadraba, Daniel - Janáček, Jiří - Filová, Eva - Lopot, F. - Paesen, R. - Fanta, O. - Jarman, A. - Nečas, A. - Ameloot, M. - Jelen, K. Calcaneal Tendon Collagen Fiber Morphometry and Aging . Microscopy and Microanalysis.. 2017, roč. 23, 5, p. 1040-1047 . IF = 2.124 [ASEP] [ doi ]
Czerneková, Michaela - Jönsson, K. I. - Chajec, Ł. - Student, S. - Poprawa, I. The structure of the desiccated Richtersius coronifer (Richters, 1903) . Protoplasma. 2017, roč. 254, 3, p. 1367-1377 . IF = 2.457 [ASEP] [ doi ]
Bačáková, Markéta - Pajorová, Julie - Stránská, D. - Hadraba, Daniel - Lopot, F. - Riedel, Tomáš - Brynda, Eduard - Žaloudková, Margit - Bačáková, Lucie . Protein nanocoatings on synthetic polymeric nanofibrous membranes designed as carriers for skin cells . International Journal of Nanomedicine. 2017, roč. 12, Feb, p. 1143-1160 . IF = 4.370 [ASEP] [ doi ]
Czerneková, Michaela - Jönsson, K. I. Mitosis in storage cells of the eutardigrade Richtersius coronifer . Zoological Journal of the Linnean Society. 2016, roč. 178, 4, p. 888-896 . IF = 2.711 [ASEP] [ doi ]
Czerneková, Michaela - Jönsson, K. I. Experimentally Induced Repeated Anhydrobiosis in the Eutardigrade Richtersius coronifer . PLoS ONE. 2016, roč. 11, 11, článku e0164062 . IF = 2.806 [ASEP] [ doi ]
Pearce, P. - Brownbill, P. - Janáček, Jiří - Jirkovská, M. - Kubínová, Lucie - Chernyavsky, I. L. - Jensen, O. E. Image-Based Modeling of Blood Flow and Oxygen Transfer in Feto-Placental Capillaries . PLoS ONE. 2016, roč. 11, 10, článku e0165369 . IF = 2.806 [ASEP] [ doi ]
Kohutiar, Matej - Ivica, Josko - Vytášek, R. - Skoumalová, A. - Illner, Jan - Šantorová, P. - Wilhelm, Jiří . Comparison of the effects of tert-butyl hydroperoxide and peroxynitrite on the oxidative damage to isolated beef heart mitochondria . Physiological Research. 2016, roč. 65, 4, p. 617-626 . IF = 1.461 [ASEP]
Filová, Eva - Jakubcová, Barbora - Danilová, I. - Kuželová Košťáková, E. - Jarošíková, T. - Chernyavskiy, Oleksandr - Hejda, J. - Handl, M. - Beznoska, Jiří - Nečas, A. - Rosina, J. - Amler, Evžen . Polycaprolactone foam functionalized with chitosan microparticles – a suitable scaffold for cartilage regeneration. Physiological Research. 2016, roč. 65, 1, p. 121-131 . IF = 1.461 [ASEP]
Wilhelm, Jiří - Vytášek, Richard - Uhlík, Jiří - Vajner, Luděk . Oxidative Stress in the Developing Rat Brain due to Production of Reactive Oxygen and Nitrogen Species . Oxidative Medicine and Cellular Longevity. 2016, roč. 2016, 2016, p. 5057610 . IF = 4.593 [ASEP] [ doi ]
Manakov, D. - Ujčíková, Hana - Pravenec, Michal - Novotný, J. Alterations in the cardiac proteome of the spontaneously hypertensive rat induced by transgenic expression of CD36 . Journal of Proteomics. 2016, roč. 145, Aug 11, p. 177-186 . IF = 3.914 [ASEP] [ doi ]
Ujčíková, Hana - Vošahlíková, Miroslava - Roubalová, Lenka - Svoboda, Petr . Proteomic analysis of protein composition of rat forebrain cortex exposed to morphine for 10 days; comparison with animals exposed to morphine and subsequently nurtured for 20 days in the absence of this drug . Journal of Proteomics. 2016, roč. 145, Aug 11, p. 11-23 . IF = 3.914 [ASEP] [ doi ]
Brejchová, Jana - Vošahlíková, Miroslava - Roubalová, Lenka - Parenti, M. - Mauri, M. - Chernyavskiy, Oleksandr - Svoboda, Petr . Plasma membrane cholesterol level and agonist-induced internalization of delta-opioid receptors; colocalization study with intracellular membrane markers of Rab family\\n . Journal of Bioenergetics and Biomembranes. 2016, roč. 48, 4, p. 375-396 . IF = 2.576 [ASEP] [ doi ]
Techniková, L. - Tunák, M. - Janáček, Jiří . Pilling evaluation of patterned fabrics based on a gradient field method . Indian Journal of Fibre & Textile Research. 2016, roč. 41, 1, p. 97-101 . IF = 0.430 [ASEP]
Kolesová, H. - Čapek, Martin - Radochová, Barbora - Janáček, Jiří - Sedmera, David . Comparison of different tissue clearing methods and 3D imaging techniques for visualization of GFP-expressing mouse embryos and embryonic hearts . Histochemistry and Cell Biology. 2016, roč. 146, 2, p. 142-152 . IF = 2.553 [ASEP] [ doi ]
Tonar, Z. - Tomášek, P. - Loskot, P. - Janáček, Jiří - Králíčková, M. - Witter, K. Vasa vasorum in the tunica media and tunica adventitia of the porcine aorta . Annals of Anatomy-Anatomischer Anzeiger. 2016, roč. 205, 2016, p. 22-36 . IF = 1.864 [ASEP] [ doi ]
Vošahlíková, Miroslava - Svoboda, Petr . Lithium - therapeutic tool endowed with multiple beneficiary effects caused by multiple mechanisms . Acta Neurobiologiae Experimentalis. 2016, roč. 76, 1, p. 1-19 . IF = 1.207 [ASEP]
Sládková, J. - Spáčilová, J. - Čapek, Martin - Tesařová, M. - Hansíková, H. - Honzík, T. - Martínek, J. - Zámečník, J. - Kostková, O. - Zeman, J. Analysis of Mitochondrial Network Morphology in Cultured Myoblasts from Patients with Mitochondrial Disorders . Ultrastructural Pathology. 2015, Vol. 39, 5, p. 340-350 . IF = 0.828 [ASEP] [ doi ]
Jirák, D. - Janáček, Jiří - Kear, B. P. A combined MR and CT study for precise quantitative analysis of the avian brain . Scientific Reports. 2015, Vol. 5, Oct 30, p. 16002 . IF = 5.228 [ASEP] [ doi ]
Roubalová, Lenka - Vošahlíková, Miroslava - Brejchová, Jana - Sýkora, Jan - Rudajev, Vladimír - Svoboda, Petr . High Efficacy but Low Potency of delta-Opioid Receptor-G Protein Coupling in Brij-58-Treated, Low-Density Plasma Membrane Fragments . PLoS ONE. 2015, Vol. 10, 8, e0135664 . IF = 3.057 [ASEP] [ doi ]
Fernandez, V. - Buffetaut, E. - Suteethorn, V. - Rage, J. C. - Tafforeau, P. - Kundrát, Martin . Evidence of Egg Diversity in Squamate Evolution from Cretaceous Anguimorph Embryos . PLoS ONE. 2015, Vol. 10, 7, e0128610 . IF = 3.057 [ASEP] [ doi ]
Wilhelm, Jiří - Ivica, Josko - Veselská, Zdeňka - Uhlík, Jiří - Vajner, Luděk . Changes in the composition of fatty acids and lipofuscin-like pigments during development of rat heart . Physiological Research. 2015, Vol. 64, 5, p. 643-651 . IF = 1.643 [ASEP]
Michálek, Jan . Total Variation-Based Reduction of Streak Artifacts, Ring Artifacts and Noise in 3D Reconstruction from Optical Projection Tomography . Microscopy and Microanalysis. 2015, Vol. 21, 6, p. 1602-1615 . IF = 1.73 [ASEP] [ doi ]
Lišková, Jana - Babchenko, Oleg - Varga, Marián - Kromka, Alexander - Hadraba, Daniel - Švindrych, Zdeněk - Burdíková, Zuzana - Bačáková, Lucie . Osteogenic cell differentiation on H-terminated and O-terminated nanocrystalline diamond films . International Journal of Nanomedicine. 2015, Vol. 10, 2015, p. 869-884 . IF = 4.32 [ASEP] [ doi ]
Kubínová, Lucie - Janáček, Jiří . Confocal stereology: an efficient tool for measurement of microscopic structures . Cell and Tissue Research. 2015, Vol. 360, 1, p. 13-28 . IF = 2.948 [ASEP] [ doi ]
Brejchová, Jana - Sýkora, Jan - Ostašov, Pavel - Merta, Ladislav - Roubalová, Lenka - Janáček, Jiří - Hof, Martin - Svoboda, Petr . TRH-receptor mobility and function in intact and cholesterol-depleted plasma membrane of HEK293 cells stably expressing TRH-R-eGFP . Biochimica Et Biophysica Acta-Biomembranes. 2015, Vol. 1848, 3, p. 781-796 . IF = 3.687 [ASEP] [ doi ]
Cvetko, E. - Čapek, Martin - Damjanovska, M. - Reina, M. A. - Eržen, I. - Stopar-Pintarič, T. The utility of three-dimensional optical projection tomography in nerve injection injury imaging . Anaesthesia. 2015, Vol. 70, 8, p. 939-947 . IF = 3.794 [ASEP] [ doi ]
Trojanová, Johana - Kulik, A. - Janáček, Jiří - Králíková, Michaela - Syka, Josef - Tureček, Rostislav . Distribution of glycine receptors on the surface of the mature calyx of Held nerve terminal . Frontiers in Neural Circuits 2014, roč. 8, oct, p. 120. ISSN 1662-5110. IF = 3.568 [ASEP] [ doi ]
Líšková, Silvia - Petrová, M. - Karen, Petr - Behuliak, Michal - Zicha, Josef . Contribution of Ca2+-Dependent Cl- Channels to Norepinephrine-Induced Contraction of Femoral Artery Is Replaced by Increasing EDCF Contribution during Ageing . BioMed Research International 2014, roč. 2014, 2014, p. 289361. ISSN 2314-6133. IF = 1.579 [ASEP] [ doi ]
Kubínová, Z. - Janáček, Jiří - Lhotáková, Z. - Kubínová, Lucie - Albrechtová, J. Unbiased estimation of chloroplast number in mesophyll cells: advantage of a genuine three-dimensional approach . Journal of Experimental Botany 2014, roč. 65, 2, p. 609-620. ISSN 0022-0957. IF = 5.526 [ASEP] [ doi ]
Janáček, Jiří - Čapek, Martin - Michálek, Jan - Karen, Petr - Kubínová, Lucie . 3D microscopic imaging and evaluation of tubular tissue architecture . Physiological Research 2014, roč. 63, suppl.1, s49-s55. ISSN 0862-8408. IF = 1.293 [ASEP]
Vaněčková, Ivana - Vokurková, Martina - Rauchová, Hana - Dobešová, Zdenka - Pecháňová, O. - Kuneš, Jaroslav - Vorlíček, Jaroslav - Zicha, Josef . Chronic antioxidant therapy lowers blood pressure in adult but not in young Dahl salt hypertensive rats: the role of sympathetic nervous system . Acta Physiologica, 2013, Vol. 208, 4, p. 340-349 . IF = 4.382 [ASEP] [ doi ]
Michálek, Jan - Čapek, Martin . A Piecewise Monotone Subgradient Algorithm for Accurate L1 -TV Based Registration of Physical Slices With Discontinuities in Microscopy . IEEE Transactions on Medical Imaging, 2013, Vol. 32, 5, p. 901-918 . IF = 4.027 [ASEP] [ doi ]
Kubínová, Lucie - Mao, X. W. - Janáček, Jiří . Blood Capillary Length Estimation from Three-Dimensional Microscopic Data by Image Analysis and Stereology . Microscopy and Microanalysis, 2013, Vol. 19, 4, p. 898-906 . IF = 2.495 [ASEP] [ doi ]
Cvetko, E. - Janáček, Jiří - Kubínová, Lucie - Eržen, I. The capillary pattern in human masseter muscle during ageing . Image Analysis and Stereology, 2013, Vol. 32, 3, p. 135-144 . IF = 0.639 [ASEP] [ doi ]
Behuliak, Michal - Pintérová, Mária - Bencze, Michal - Petrová, M. - Líšková, Silvia - Karen, Petr - Kuneš, Jaroslav - Vaněčková, Ivana - Zicha, Josef . Ca2+ sensitization and Ca2+ entry in the control of blood pressure and adrenergic vasoconstriction in conscious Wistar-Kyoto and spontaneously hypertensive rats . Journal of Hypertension, 2013, Vol. 31, 10, p. 2025-2035 . IF = 3.806 [ASEP] [ doi ]
Šepitka, J. - Lukeš, J. - Staněk, L. - Filová, Eva - Burdíková, Zuzana - Řezníček, J. Nanoindentation of intervertebral disc tissues localised by SHG imaging . Computer methods in biomechanics and biomedical engineering, 2012, Vol. 15, Suppl.1, p. 335-336 . IF = 1.393 [ASEP] [ doi ]
Vohník, Martin - Burdíková, Zuzana - Wilkinson, D. M. Testate Amoebae Communities in the Rhizosphere of Rhododendron ponticum (Ericaceae) in an Evergreen Broadleaf Forest in Southern Spain . Acta Protozoologica, 2012, Vol. 51, Sp. iss.3, p. 259-269 . IF = 0.984 [ASEP] [ doi ]
Skalský, I. - Szárszoi, O. - Filová, Elena - Pařízek, Martin - Lytvynets, Andriy - Malušková, J. - Lodererová, A. - Brynda, Eduard - Lisá, Věra - Burdíková, Zuzana - Čapek, Martin - Pirk, J. - Bačáková, Lucie . A perivascular system releasing sirolimus prevented intimal hyperplasia in a rabbit model in a medium-term study . International Journal of Pharmaceutics, 2012, Vol. 427, 2, p. 311-319 . IF = 3.458 [ASEP] [ doi ]
Lhotáková, Z. - Urban, Otmar - Dubánková, M. - Cvikrová, Milena - Tomášková, Ivana - Kubínová, Lucie - Zvára, K. - Marek, Michal V. - Albrechtová, J. The impact of long-term CO2 enrichment on sun and shade needles of Norway spruce (Picea abies): Photosynthetic performance, needle anatomy and phenolics accumulation . Plant Science, 2012, 188-189, JUN, p. 60-70 . IF = 2.922 [ASEP] [ doi ]
Jirkovská, M. - Kučera, T. - Kaláb, J. - Jadrníček, M. - Niedobová, V. - Janáček, Jiří - Kubínová, Lucie - Moravcová, M. - Žižka, Z. - Krejčí, V. The branching pattern of villous capillaries and structural changes of placental terminal villi in type 1 diabetes mellitus . Placenta, 2012, Vol. 33, 5, p. 343-351 . IF = 3.117 [ASEP] [ doi ]
Janáček, Jiří - Kreft, M. - Čebašek, V. - Eržen, I. Correcting the axial shrinkage of skeletal muscle thick sections visualized by confocal microscopy . Journal of Microscopy, 2012, Vol. 246, 2, p. 107-112 . IF = 1.633 [ASEP] [ doi ]
Hudcovic, Tomáš - Kolínská, Jiřina - Klepetář, Jan - Štěpánková, Renata - Řezanka, Tomáš - Šrůtková, Dagmar - Schwarzer, Martin - Erban, V. - Du, Zhengyu - Wells, J. M. - Hrnčíř, Tomáš - Tlaskalová-Hogenová, Helena - Kozáková, Hana . Protective effect of Clostridium tyrobutyricum in acute dextran sodium sulphate-induced colitis: differential regulation of tumour necrosis factor-α and interleukin-18 in BALB/c and severe combined immunodeficiency mice . Clinical and Experimental Immunology, 2012, Vol. 167, 2, p. 356-365 . IF = 3.409 [ASEP] [ doi ]
Cvetko, E. - Karen, Petr - Eržen, I. Wearing of complete dentures reduces slow fibre and enhances hybrid fibre fraction in masseter muscle . Journal of Oral Rehabilitation, 2012, Vol. 39, 8, p. 608-614 . IF = 2.344 [ASEP] [ doi ]
Cvetko, E. - Karen, Petr - Eržen, I. Myosin heavy chain composition of the human sternocleidomastoid muscle . Annals of Anatomy-Anatomischer Anzeiger, 2012, Vol. 194, 5, p. 467-472 . IF = 1.960 [ASEP] [ doi ]
Cvetko, E. - Karen, Petr - Janáček, Jiří - Kubínová, Lucie - Plasencia, A.L. - Eržen, I. Human Masseter Muscle Fibers From the Elderly Express Less Neonatal Myosin Than Those of Young Adults . Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology, 2012, Vol. 295, 8, p. 1364-1372 . IF = 1.343 [ASEP] [ doi ]
Burdíková, Zuzana - Čapek, Martin - Švindrych, Zdeněk - Gryndler, M. - Kubínová, Lucie - Holcová, K. Ecology of Testate Amoebae in the Komořany Ponds in the Vltava Basin . Microbial Ecology, 2012, Vol. 64, 1, p. 117-130 . IF = 3.277 [ASEP] [ doi ]
Váchová, Libuše - Šťovíček, V. - Hlaváček, Otakar - Chernyavskiy, Oleksandr - Štěpánek, L. - Kubínová, Lucie - Palková, Z. Flo11p, drug efflux pumps, and the extracellular matrix cooperate to form biofilm yeast colonies . Journal of Cell Biology, 2011, Vol. 194, 5, p. 679-687 . IF = 10.264 [ASEP] [ doi ]
Vohník, Martin - Burdíková, Zuzana - Vyhnal, Aleš - Koukol, O. Interactions Between Testate Amoebae and Saprotrophic Microfungi in a Scots Pine Litter Microcosm . Microbial Ecology, 2011, Vol. 61, 3, p. 660-668 . IF = 2.912 [ASEP] [ doi ]
Skalský, I. - Filová, Elena - Szárszoi, O. - Pařízek, Martin - Lytvynets, Andriy - Malušková, J. - Lodererová, A. - Brynda, Eduard - Lisá, Věra - Burdíková, Zuzana - Čapek, Martin - Pirk, J. - Bačáková, Lucie . A periadventitial sirolimus-releasing mesh decreased intimal hyperplasia in a rabbit model . Physiological Research, 2011, Vol. 60, 3, p. 585-588 . IF = 1.555 [ASEP]

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People

RNDr. Jiří Janáček, PhD.
Head of the Laboratory
Ing. Martin Čapek, PhD.
Senior Researcher (part-time)
RNDr. Barbora Radochová, PhD.
Senior Researcher
Ing. Mgr. Daniel Hadraba, Ph.D.
Postdoctoral Researcher
MUDr. Petr Karen, CSc.
Senior Researcher
 

Mgr. David Kala

PhD Student

 

Michaela Vodičková

PhD Student

Mgr. David Vondrášek

Technician

Mgr. Zuzana Kubínová

Technician

Ing. Jitka Čočková
Project Manager
 

Bc. Jonáš Kaufman

Research Assistant

 

Mgr. Diana Siswartonová

Research Assistant

 

Ivana Švandová

Research Assistant

 

Petr Zvolánek

Research Assistant

 

 

Biochemistry of Membrane Receptors

Doc. RNDr. Petr Svoboda, DrSc.
Senior researcher
RNDr. Lenka Roubalová, PhD.
Senior researcher
RNDr. Hana Ujčíková, PhD.
Senior researcher
Ing. Miroslava Vošahlíková, PhD.
Senior researcher
Mgr. Jana Brejchová, PhD.
Senior researcher
  Mgr. Kristina Cechová
PhD student