Intranet

Shared Equipment

Shared Equipment
For equipment booking use our on-line reservation system (requires registration).
 

Microscopy facility

  Equipment Year Dept Location Use for Manager Operator
Bruker Ultima IntraVital 2018 60 DAI upright multiphoton microscope for in vivo and in vitro imaging Mgr. Daniel Hadraba Mgr. David Vondrášek
Leica SP8 AOBS WLL MP confocal 2015 60 DAI 10 Inverted laser scanning and multiphoton microscope Mgr. Daniel Hadraba Mgr. David Vondrášek
Leica SP2 AOBS 2007 60 DAI 9 Inverted laser scanning and fluorescence microscope Mgr. Daniel Hadraba Mgr. David Vondrášek
Leica SPE confocal 2007 60 DAI 9 Upright laser scanning and fluorescence microscope Mgr. Daniel Hadraba Mgr. David Vondrášek
Optical projection tomography 2011 60 DAI 2 3D imaging of transparent samples Radochová Barbora, RNDr., Ph.D.  
CellVizio Optic fibre confocal microscopy 2015 33 D 050 Optic fibre confocal microscope capable of fluorescence imaging from surface to deep tissues in whole animal. See more. Otáhal Jakub doc. MUDr. PhD Mgr. David Kala
  Neuroimage - stereology microscope 2010 33 D 048 Neuroimage (MBF Bioscience Kubová Hana doc. Dr. DrSc.  
  Leica LMD 6000 Laser capture microdissector 2008 21 D 239 Allows to identify and dissect microareas from tissue or cell smear. Martin Vodička Mgr.  
Carv II system 2013 32 D 048 Spinning disc confocal microscope Hadraba Daniel Mgr. Mgr. David Vondrášek

Proteomic facility

For more information, you can also directly check the ProteoLab website.

Equipment Year Dept Location Use for Manager Operator
Mass spectrometer 2005 22 E 305 HPLC and mass spectrometry analysis of physiologically important compounds Mikšík Ivan prof. Ing. DrSc.  
Capillary electrophoresis Agilent 7100  2012 22 E 201 Separation instrument for analysis of a broad spectrum of compounds Mikšík Ivan prof. Ing. DrSc. Mgr. Pavla Sedláková, PhD
2D electrophoresis and Bruker MaXis MS 2009 22 E 306 Proteomic analysis Mikšík Ivan prof. Ing. DrSc. Ing. Statis Pataridis

Metabolomic facility

Equipment Year Dept Location Use for Manager Operator
Qtrap 5500 mass spectrometer 2012 17 E 218 metabolomics, lipidomics Kuda Ondřej RNDr. Ph.D. Ivana Brabcová, Ph.D.

 


 

CentriVap Refrigerated Vacuum Concentrator

2016 17 E 218 metabolomics Kuda Ondřej RNDr. Ph.D.  

Whole body phenotyping

  Equipment Year Dept Location Use for Manager Operator
                 
 

Behavioral analysis

           
  PhenoWorld - automatic modular system 2015 32 G Modular system for complex behavioral analyses Kubík Štěpán, RNDr. PhD.  
  Multi conditioning system 2011 32 D 037 System accommodating nine standard conditioning paradigms for the evaluation of learning, memory, emotion and stress-related behaviors in mice and rats. RNDr. Štěpán Kubík, Ph.D.  
  Phenotyper - system for behavioral analysis 2013 33 D 011 PhenoTyper (Noldus) for 8 rats. System is designed for automatated behavioral analysis in instrumented observation cage.   Kubová Hana doc. Dr. DrSc.  
  Hematology Analyzer scil Vet abc Plus+ 2016 41 E 017 Analytical Instrument to determine haematological parameters from whole blood   RNDr. Světlana. Žufanová
               
 

CT/PET

           
microCT/PET Albira 2013 96 E 013 In vivo imaging of small laboratory animals Roman Liška  
                 
 

Echocardiography

           
  GE-VIVID 7 echocardiography 2010 80 D302b Non-invasive assessment of heart geometry and function Papoušek František Ing. CSc.  
                 
 

Blood pressure

           
  Telemetric monitoring of blood pressure 2013 25 D Long term telemetric monitoring of blood pressure and sympathetic activity Vaněčková Ivana RNDr. CSc.  
                 
 

Metabolism

           
  Indirect calorimetry INCA Somedic 2012 17 G 008 Indirect calorimetry for mice and rats Kristína Bardová Mgr.  

Mitochondrial energetics

Equipment Year Dept Location Use for Manager Operator
Oroboros Oxygraph 2k 2013 13+17 DAII 108 Three setups for measurement of cellular/mitochondrial respiration. One with fluorescent attachment for ROS/ATP monitoring Pecina Petr, Mgr. PhD  
Seahorse Xfe-24 alanyzer 2014 13 DAII 228 Plate based system for measurement of respiration and glycolytic activity of cells Mráček Tomáš, RNDr. PhD  

Molecular biology facility

Equipment Year Dept Location Use for Manager Operator
Agilent RNA analyzer 2004 21 D 224 Provides sizing, quantitation and quality control of DNA, RNA, proteins and cells on a single platform. Ergang Peter Ing. PhD  
Nanodrop spectrophotometer 2007 21 D 224 Measurement of nucleic acid concentration and purity and fluorescent dye labeled proteins. Ergang Peter Ing. PhD  
Light Cycler 480 real-time PCR 2009 17 D 224 Real-time PCR system    
ViiA 7 real-time PCR 2012 21 D 224 Real-time PCR system with HRM possibility Ergang Peter Ing. PhD  

Plate-readers

Equipment

Year

Dept

Location

Use for

Manager

Operator

Microbeta counter 2004 14 D 121 scintillation counting in a plate format Doležal Vladimír MUDr. DrSc  
Reader Tecan infinity M200 2007 13 DAII 112 Luminescence, Fluorescence (top reading), UV-absorbance (UV-VIS) plate-reader with injectors  Mráček Tomáš, RNDr. PhD  
Reader Victor X4 2011 14 D 121 Luminescence, Fluorescence Intensity (both top and bottom reading), UV-absorbance (UV-VIS) and Time-Resolved Fluorescence technologies Doležal Vladimír MUDr. DrSc  
Reader Synergy HT 2008 16 D 324 single-channel absorbance, fluorescence (400/30, 460/40, 485/20, 508/20, 516/20, 528/20, 560/15, 590/20) and luminiscence microplate reader. Zimmermannová Olga Ing. PhD  

ULTRACENTRIFUGES and ROTORS

Equipment  

Year

Dept. Location Use for

Manager

Operator
Beckman Optima XL-100K 2001 60 D 024 floor model, max speed 100,000 rpm, rotors: 35 (retired), 45 Ti, 50.3 Ti, 70 Ti, 70.1 Ti, 90 Ti, NVT 90, SW 28, SW 41 Ti Petr Svoboda  
Beckman Optima L-90 1996 13 DaII 114 floor model, max speed 90,000 rpm, rotors: same as for Optima XL-100K Marek Vrbacky  
Beckman Optima MAX 130K 2007 60 E 318 benchtop model, max speed 130,000 rpm (1,019,000g with MLA-130 rotor), rotors: MLA-80, MLA-130, TLA-100, TLA-110, MLN-80 Petr Svoboda  
Beckman L8-55 1983 75 G 044 floor model, rotors: same as for Optima XL-100K Tomáš Špaček  
Sorvall RC M100 1991 60 D 121

floor model, rotors: RP100AT3, RP100AT4, RP100VT

Petr Svoboda

 
Further info on Ultracentrifuges, Rotors, and Tubes