This year marks the 65th anniversary of the founding of the Institute of Physiology of the Czech Academy of Sciences. On June 12, 2019, both current and former employees of the Institute and a number of guests celebrated this special event. All the living directors of the Institute took turns in the lecture hall (in the order of their terms in office: Zdeněk Drahota, Bohuslav Ošťádal, Pavel Mareš, Jaroslav Kuneš, Lucie Kubínová, and the current director, Jan Kopecký) and shared their memories of important moments in the Institute's history. Helena Illnerová, the President of the Czech Academy of Sciences in 2001-2005, also reminisced about the beginnings of her scientific career and the atmosphere of that time. The celebrations culminated with an informal garden party, which was also attended by the President of the Academy, Eva Zažímalová, and the Vice President, Zdeněk Havlas.