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INSTITUTE OF PHYSIOLOGY CAS

Cutting-edge science for health

Laboratory of Translational Metabolism

Laboratory of Translational Metabolism Translational metabolism: Bridging the gap between research and medicine in metabolism

Our research involves applying mass spectrometry-based techniques to perform metabolomic and proteomic analysis of various biological samples. We develop and use liquid chromatography and capillary electrophoresis–mass spectrometry-based (LC–MS, CE–MS) methods for in-depth characterization and quantification of complex lipids (lipidome), polar metabolites (metabolome), various exogenous compounds such as food components and drugs (exposome), and proteins (proteome). We also focus on improving data processing, automated data curation, statistical methods, and visualization of omics data.

We have successfully integrated LC–MS methods for targeted and untargeted analysis of lipids, polar metabolites, and exposome compounds (LIMeX) for diverse sample types and used them for studies focused on cardiovascular diseases, type 2 diabetes, lipogenesis, circadian rhythms, and drug adherence.

We have also investigated the proteome of human teeth and saliva, their link to tooth decay as well as proteins to tissue architecture at clubfoot and pulmonary hypertension. Posttranslational modifications of proteins are studied in connection to aging and disease of organism.

PhD Program 2021 - Call for Applications

1. Mass spectrometry-based metabolomics of biological systems

Untargeted metabolomics methods focus on the analysis of all the detectable metabolites in a sample, including chemical unknowns. Liquid chromatography coupled to mass spectrometry (LC-MS) is the preferred technique in metabolomics permitting effective compound separations and detection. However, up to 80% of all detected signals from untargeted profiling represent unknown metabolites. Such a big obstacle in biomedical and biological research hinders meaningful biochemical and pathway interpretations. The PhD project aims to focus on (i) increasing the coverage of spectral libraries used for metabolite annotation, (ii) applying programs for the prediction of ‘unknown’ metabolites, and (iii) using bioinformatics tools for visualization and interpretation of the data obtained within metabolomics studies (e.g., type 2 diabetes, circadian rhythms). The work will be conducted at the Institute of Physiology CAS and financially supported by various grants (GACR, MSMT, AZV).

Supervisor: T. Čajka (tomas.cajka@fgu.cas.cz)

2. Liquid chromatography–mass spectrometry for metabolomics and lipidomics studies 

Over the last decade, mass spectrometry-based metabolomics and lipidomics have become key disciplines for the comprehensive profiling of polar metabolites and complex lipids in biological systems. Liquid chromatography–mass spectrometry (LC–MS) is the preferred technique in metabolomics and lipidomics permitting effective compound separations and detection. However, there is still a lack of sufficient data on the metabolome and lipidome characterizing biofluids (i.e., plasma, serum, urine) and tissues, which can be easily accessible and reused at any time for future studies. The PhD project aims to focus on (i) merging targeted and untargeted metabolomics and lipidomics methods, (ii) standardization of methods, and (iii) extending the breadth and scope of methods for metabolomics and lipidomics studies (e.g., type 2 diabetes, circadian rhythms). The work will be conducted at the Institute of Physiology CAS and financially supported by various grants (GACR, MSMT, AZV).

Supervisor: T. Čajka (tomas.cajka@fgu.cas.cz)

 

Main topics

  • Simple, fast, and robust protocols for metabolomics and proteomics projects
  • Processing, curation, statistical analysis, and visualization of metabolomics data
  • New separation methods (LC–MS, capillary electromigration techniques incl. CE–MS) for physiological research
  • Metabolomics for studying circadian rhythms
  • Metabolomics and proteomics for discovering new biomarkers of human health
  • Predictive role of proteomic composition of teeth and saliva in the etiology of tooth decay
  • Analysis of extracellular matrix disorders: from proteins to tissue architecture at idiopathic pes equinovarus
  • Mechanisms of pulmonary arterial wall remodeling during pulmonary hypertension development
  • Posttranslational modifications of proteins during aging and disease (e.g. diabetes mellitus)

Projects

Hydrogen-deuterium exchange mass spectrometry (HDX-MS) to identify unknown metabolites detected by untargeted metabolomics (21-00477S)

  • PI: T. Cajka, Laboratory of Translational Metabolism

Identification of factors influencing adherence to pharmacotherapy in patients with type 2 diabetes using untargeted metabolomics (ENIGMA) (NU20-01-00186)

  • PI: T. Cajka, Laboratory of Translational Metabolism

Metabolomics 2.0: Comprehensive analytical methods and bioinformatics tools for the study of circadian rhythms (LTAUSA19124)

  • PI: T. Cajka, Laboratory of Translational Metabolism

Mapping of the rat circadian metabolome in brain and peripheral tissues during embryonic to postnatal development (20-21114S)

  • PI: T. Cajka, Laboratory of Translational Metabolism

Role of adipose tissue metabolism in propensity to obesity: UCP1-independent mechanisms (19-02411S)

  • Team member: T. Cajka, Laboratory of Translational Metabolism
  • PI: J. Kopecky, Laboratory of Adipose Tissue Biology

The role of epicardial fat, subclinical inflammation and novel lipid signalling molecules in the onset and development of heart failure (NV19-02-00118)

  • Team member: T. Cajka, Laboratory of Translational Metabolism
  • PI: M. Haluzik, Institute of Clinical and Experimental Medicine, Prague; co-PI: O. Kuda, Laboratory of Metabolism of Bioactive Lipids

Changes of transcriptome during early postnatal development in humans: impact of premature birth on control of energy metabolism (NU20-07-00026)

  • Team member: T. Cajka, Laboratory of Translational Metabolism
  • PI: J. Kopecky, Laboratory of Adipose Tissue Biology

Bone marrow adipose tissue (BMAT) as a novel adipose depot in the regulation of the whole body metabolism and bone homeostasis (20-03899S)

  • Team member: T. Cajka, Laboratory of Translational Metabolism
  • PI: M. Tencerova, Laboratory of Molecular Physiology of Bone

Studying metabolic flexibility of bone marrow adipose tissue in obesity (EFSD/Novo Nordisk Foundation Future Leaders Award)

  • Team member: T. Cajka, Laboratory of Translational Metabolism
  • PI: M. Tencerova, Laboratory of Molecular Physiology of Bone

Affinity capillary electrokinetic methods for selective analysis of biopolymers and metabolites and for study of their interactions (20-03899S)

  • co-PI: I. Miksik, Laboratory of Translational Metabolism
  • PI: V. Kašička, Institute of Organic Chemistry and Biochemistry of the CAS, Prague

Bioartificial cardiovascular patches and vessels from porcine collagen reinforced with nano/microfibers using stem cells and dynamic culture (NV19-02-00068)

  • Team member: A. Eckhardt, Laboratory of Translational Metabolism
  • PI: J. Pirk, Institute for Clinical and Experimental Medicine, Prague

Projects

Achievements

The Power List 2018 -- The Fab Forty

Assoc. Prof. Tomas Cajka has been ranked among 40 best scientists under 40 years in analytical chemistry. The ranking of the 40 most successful scientists was published in the October issue of The Analytical Scientist. More

The Best Poster Award

Dr. Tomáš Novotný was awarded “The Lester Lowe Award” at the 40th World Orthopaedic Congerss, Muscat, Oman (2019, organizer: SICOT) for the poster “Histological comparison of clubfoot deformity and Dupuytren's disease fibroproliferation”. More

Publications

Sistilli; Gabriella - Kalendová; Veronika - Čajka; Tomáš - Irodenko; Ilaria - Bardová; Kristina - Oseeva; Marina - Žáček; P. - Kroupová; Petra - Horáková; Olga - Lackner; K. - Gastaldelli; A. - Kuda; Ondřej - Kopecký; Jan - Rossmeisl; Martin . Krill Oil Supplementation Reduces Exacerbated Hepatic Steatosis Induced by Thermoneutral Housing in Mice with Diet-Induced Obesity . Nutrients. 2021; 13(2)); 437 . IF = 4.546 [ASEP] [ doi ]
Novotný; T. - Eckhardt; Adam . Response to: "Quantity;" "Quality" and "Distribution Pattern" of Neo Vascular System: Is it the Time to Come Back to Aristotle Categories? . Journal of Pediatric Orthopaedics. 2021; 41(2); E199-E200 . IF = 1.909 [ASEP] [ doi ]
Filová; Elena - Steinerová; Marie - Trávníčková; Martina - Knitlová; Jarmila - Musílková; Jana - Eckhardt; Adam - Hadraba; Daniel - Matějka; Roman - Pražák; Šimon - Štěpanovská; Jana - Kučerová; Johanka - Riedel; Tomáš - Brynda; Eduard - Lodererová; A. - Honsová; E. - Pirk; J. - Koňařík; M. - Bačáková; Lucie . Accelerated in vitro recellularization of decellularized porcine pericardium for cardiovascular grafts . Biomedical Materials. 2021; 16(2)); 025024 . IF = 3.174 [ASEP] [ doi ]
Brejchová; Kristýna - Radner; F. P. W. - Balas; L. - Palůchová; Veronika - Čajka; Tomáš - Chodounská; Hana - Kudová; Eva - Schratter; M. - Schreiber; R. - Durand; T. - Zechner; R. - Kuda; Ondřej . Distinct roles of adipose triglyceride lipase and hormone-sensitive lipase in the catabolism of triacylglycerol estolides . Proceedings of the National Academy of Sciences of the United States of America. 2021; 118(2)); e2020999118 . IF = 9.412 [ASEP] [ doi ]
Vošahlíková; Miroslava - Roubalová; Lenka - Cechová; Kristína - Kaufman; Jonáš - Musil; Stanislav - Mikšík; Ivan - Alda; M. - Svoboda; Petr . Na+/K+-ATPase and lipid peroxidation in forebrain cortex and hippocampus of sleep-deprived rats treated with therapeutic lithium concentration for different periods of time . Progress in Neuro-Psychopharmacology & Biological Psychiatry. 2020; 102(Aug 30)); 109953 . IF = 4.361 [ASEP] [ doi ]
Vajrychová; M. - Stráník; J. - Pimková; K. - Barman; M. - Kukla; R. - Zedníková; Petra - Bolehovská; R. - Plíšková; L. - Hornychová; H. - Andrýs; C. - Tambor; V. - Lenčo; J. - Jacobsson; B. - Kacerovský; M. Comprehensive proteomic investigation of infectious and inflammatory changes in late preterm prelabour rupture of membranes . Scientific Reports. 2020; 10(1)); 17696 . IF = 3.998 [ASEP] [ doi ]
Ujčíková; Hana - Cechová; Kristína - Jágr; Michal - Roubalová; Lenka - Vošahlíková; Miroslava - Svoboda; Petr . Proteomic analysis of protein composition of rat hippocampus exposed to morphine for 10 days; comparison with animals after 20 days of morphine withdrawal . PLoS ONE. 2020; 15(4)); e0231721 . IF = 2.740 [ASEP] [ doi ]
Tsugawa; H. - Ikeda; K. - Takahashi; M. - Satoh; A. - Mori; Y. - Uchino; H. - Okahashi; N. - Yamada; Y. - Tada; I. - Bonini; P. - Higashi; Y. - Okazaki; Y. - Zhou; Z. - Zhu; Z.-J. - Koelmel; J. - Čajka; Tomáš - Fiehn; O. - Saito; K. - Arita; Mas. - Arita; Mak. A lipidome atlas in MS-DIAL 4 . Nature Biotechnology. 2020; 38(10); 1159-1163 . IF = 36.553 [ASEP] [ doi ]
Svobodová; Jana - Mikšík; Ivan . Open-tubular capillary electrochromatographic application of a sol-gel matrix with chilli peppers; garlic; or synthetic additives . Journal of Separation Science. 2020; 43(18); 3691-3701 . IF = 2.878 [ASEP] [ doi ]
Palůchová; Veronika - Vik; A. - Čajka; Tomáš - Březinová; Marie - Brejchová; Kristýna - Bugajev; Viktor - Dráberová; Lubica - Dráber; Peter - Burešová; Jana - Kroupová; Petra - Bardová; Kristina - Rossmeisl; Martin - Kopecký; Jan - Hansen; T. V. - Kuda; Ondřej . Triacylglycerol-Rich Oils of Marine Origin are Optimal Nutrients for Induction of Polyunsaturated Docosahexaenoic Acid Ester of Hydroxy Linoleic Acid (13-DHAHLA) with Anti-Inflammatory Properties in Mice . Molecular Nutrition & Food Research. 2020; 64(11)); 1901238 . IF = 5.309 [ASEP] [ doi ]
Palůchová; Veronika - Oseeva; Marina - Březinová; Marie - Čajka; Tomáš - Bardová; Kristina - Adamcová; Kateřina - Žáček; Petr - Brejchová; Kristýna - Balas; L. - Chodounská; Hana - Kudová; Eva - Schreiber; R. - Zechner; R. - Durand; T. - Rossmeisl; Martin - Abumrad; N. A. - Kopecký; Jan - Kuda; Ondřej . Lipokine 5-PAHSA is Regulated by Adipose Triglyceride Lipase and Primes Adipocytes for de novo Lipogenesis in Mice . Diabetes. 2020; 69(3); 300-312 . IF = 7.720 [ASEP] [ doi ]
Novotný; Tomáš - Eckhardt; Adam - Knitlová; Jarmila - Doubková; Martina - Ošťádal; M. - Uhlík; J. - Musílková; Jana . Increased Microvessel and Arteriole Density in the Contracted Side of the Relapsed Clubfoot . Journal of Pediatric Orthopaedics. 2020; 40(10); 592-596 . IF = 1.909 [ASEP] [ doi ]
Nemet; I. - Saha; P. P. - Gupta; N. - Zhu; W. - Romano; K. A. - Skye; S. M. - Čajka; Tomáš - Mohan; M. L. - Li; L. - Wu; Y. - Funabashi; M. - Ramer-Tait; A. E. - Prasad; S. V. N. - Fiehn; O. - Rey; F. E. - Tang; W.H.W. - Fischbach; M. A. - DiDonato; J. A. - Hazen; S.L. A Cardiovascular Disease-Linked Gut Microbial Metabolite Acts via Adrenergic Receptors . Cell. 2020; 180(5); 862-877 . IF = 38.637 [ASEP] [ doi ]
Mikšík; Ivan - Kubinová; Šárka - Morvan; Marine - Výborný; Karel - Tatar; A. - Král; V. - Záruba; K. - Sýkora; D. Analysis of Chondroitin/Dermatan Sulphate Disaccharides Using High-Performance Liquid Chromatography . Separations. 2020; 7(3)); 49 . IF = 1.900 [ASEP] [ doi ]
Kulhavá; Lucie - Eckhardt; Adam - Pataridis; Statis - Foltán; R. - Mikšík; Ivan . Proteomic Analysis of Whole Saliva in Relation to Dental Caries Resistance . Folia Biologica. 2020; 66(2); 72-80 . IF = 0.691 [ASEP]
Janovská; Petra - Melenovský; V. - Svobodová; Michaela - Havlenová; T. - Kratochvílová; H. - Haluzík; M. - Hošková; E. - Pelikánová; T. - Kautzner; J. - Monzo; L. - Jurcová; I. - Adamcová; Kateřina - Leňková; Lucie - Burešová; Jana - Rossmeisl; Martin - Kuda; Ondřej - Čajka; Tomáš - Kopecký; Jan . Dysregulation of epicardial adipose tissue in cachexia due to heart failure: the role of natriuretic peptides and cardiolipin . Journal of Cachexia Sarcopenia and Muscle. 2020; 11(6); 1614-1627 . IF = 9.802 [ASEP] [ doi ]
Filová; Elena - Staňková; Ľubica - Eckhardt; Adam - Svobodová; Jana - Musílková; Jana - Pala; J. - Hadraba; Daniel - Brynda; Eduard - Koňařík; M. - Pirk; J. - Bačáková; Lucie . Modification of Human Pericardium by Chemical Crosslinking . Physiological Research. 2020; 69(1); 49-59 . IF = 1.655 [ASEP] [ doi ]
Březinová; Marie - Čajka; Tomáš - Oseeva; Marina - Štěpán; M. - Daďová; K. - Rossmeislová; L. - Matouš; M. - Šiklová; M. - Rossmeisl; Martin - Kuda; Ondřej . Exercise training induces insulin-sensitizing PAHSAs in adipose tissue of elderly women . Biochimica Et Biophysica Acta-Molecular and Cell Biology of Lipids. 2020; 1865(2)); 158576 . IF = 4.519 [ASEP] [ doi ]
Bardová; Kristina - Funda; Jiří - Pohl; Radek - Čajka; Tomáš - Hensler; Michal - Kuda; Ondřej - Janovská; Petra - Adamcová; Kateřina - Irodenko; Ilaria - Leňková; Lucie - Zouhar; Petr - Horáková; Olga - Flachs; Pavel - Rossmeisl; Martin - Colca; J. - Kopecký; Jan . Additive Effects of Omega-3 Fatty Acids and Thiazolidinediones in Mice Fed a High-Fat Diet: Triacylglycerol/Fatty Acid Cycling in Adipose Tissue . Nutrients. 2020; 12(12)); 3737 . IF = 4.546 [ASEP] [ doi ]
Žaloudíková; M. - Eckhardt; Adam - Vytášek; R. - Uhlík; J. - Novotný; T. - Bačáková; Lucie - Musílková; Jana - Hampl; V. Decreased collagen VI in the tunica media of pulmonary vessels during exposure to hypoxia: a novel step in pulmonary arterial remodeling . Pulmonary Circulation. 2019; 9(3)); 2045894019860747 . IF = 2.205 [ASEP] [ doi ]
Tsugawa; H. - Satoh; A. - Uchino; H. - Čajka; Tomáš - Arita; Mak. - Arita; Mas. Mass Spectrometry Data Repository Enhances Novel Metabolite Discoveries with Advances in Computational Metabolomics . Metabolites. 2019; 9(6)); 119 . IF = 4.097 [ASEP] [ doi ]
Sechovcová; Hana - Kulhavá; Lucie - Fliegerová; Kateřina - Trundová; Mária - Morais; Daniel - Mrázek; Jakub - Kopečný; Jan . Comparison of enzymatic activities and proteomic profiles of Butyrivibrio fibrisolvens grown on different carbon sources . Proteome Science. 2019; 17(JUN 1)); 2 . IF = 2.811 [ASEP] [ doi ]
Pataridis, Statis - Romanov, O. - Mikšík, Ivan . Identification of short-chain poly-3-hydroxybutyrates in Saiga horn extracts using LC-MS/MS . Journal of Separation Science 2019, 42(4), 797-808 . IF = 2.878 [ASEP] [ doi ]
Mikšík, Ivan . Coupling of CE-MS for protein and peptide analysis . Journal of Separation Science 2019, 42(1), 385-397 . IF = 2.878 [ASEP] [ doi ]
Jágr, Michal - Ergang, Peter - Pataridis, Statis - Kolrosová, M. - Bartoš, M. - Mikšík, Ivan . Proteomic analysis of dentin-enamel junction and adjacent protein-containing enamel matrix layer of healthy human molar teeth . European Journal of Oral Sciences 2019, 127(2), 112-121 . IF = 2.220 [ASEP] [ doi ]
Gvoždíková Javůrková; V. - Pokorná; M. - Mikšík; Ivan - Tůmová; E. Concentration of egg white antimicrobial and immunomodulatory proteins is related to eggshell pigmentation across traditional chicken breeds . Poultry Science. 2019; 98(12); 6931-6941 . IF = 2.659 [ASEP] [ doi ]
Fan, S. - Kind, T. - Čajka, Tomáš - Hazen, S.L. - Tang, W.H.W. - Kaddurah-Daouk, R. - Irvin, M. R. - Arnett, D. K. - Barupal, D. K. - Fiehn, O. Systematic Error Removal Using Random Forest for Normalizing Large-Scale Untargeted Lipidomics Data . Analytical Chemistry 2019, 91(5), 3590-3596 . IF = 6.785 [ASEP] [ doi ]
Eckhardt, Adam - Novotný, T. - Doubková, Martina - Hronková, Lucia - Vajner, L. - Pataridis, Statis - Hadraba, Daniel - Kulhavá, Lucie - Plencner, Martin - Knitlová, Jarmila - Lišková, Jana - Uhlík, J. - Žaloudíková, M. - Vondrášek, David - Mikšík, Ivan - Ošťádal, M. Novel contribution to clubfoot pathogenesis: The possible role of extracellular matrix proteins . Journal of Orthopaedic Research 2019, 37(3), 769-778 . IF = 2.728 [ASEP] [ doi ]
Čepková Hlásná, P. - Jágr, Michal - Viehmannová, I. - Dvořáček, V. - Huansi, D. C. - Mikšík, Ivan . Diversity in Seed Storage Protein Profile of Oilseed Crop Plukenetia volubilis from Peruvian Amazon . International Journal of Agriculture and Biology 2019, 21(3), 679-688 . IF = 0.822 [ASEP]
Čajka; Tomáš . Towards Merging Targeted and Untargeted Analysis of the Lipidome; Metabolome; and Exposome . LC GC Europe. 2019; 32(6); 314-316 . IF = 1.482 [ASEP]
Angelisová, Pavla - Ballek, Ondřej - Sýkora, Jan - Benada, Oldřich - Čajka, Tomáš - Pokorná, Jana - Pinkas, Dominik - Hořejší, Václav . The use of styrene-maleic acid copolymer (SMA) for studies on T cell membrane rafts . Biochimica Et Biophysica Acta-Biomembranes 2019, 1861(1), 130-141 . IF = 3.411 [ASEP] [ doi ]
Peševski, Živorad - Kvasilová, A. - Stopková, T. - Naňka, O. - Drobná Krejčí, Eliška - Buffinton, Ch. - Kočková, Radka - Eckhardt, Adam - Sedmera, David . Endocardial Fibroelastosis is Secondary to Hemodynamic Alterations in the Chick Embryonic Model of Hypoplastic Left Heart Syndrome . Developmental Dynamics 2018, roč. 247, 3, p. 509-520 . IF = 2.852 [ASEP] [ doi ]
Mikšík, Ivan - Pataridis, Statis - Eckhardt, Adam - Sedmera, David . Analysis of Siamese Crocodile (Crocodylus siamensis) Eggshell Proteome . Protein Journal 2018, roč. 37, 1, p. 21-37 . IF = 1.029 [ASEP]
Marschner, Karel - Musil, Stanislav - Mikšík, Ivan - Dědina, Jiří . Investigation of hydride generation from arsenosugars - Is it feasible for speciation analysis? . Analytica Chimica Acta 2018, roč. 1008, MAY, p. 8-17 . IF = 5.256 [ASEP] [ doi ]
Kulhavá, Lucie - Eckhardt, Adam - Pataridis, Statis - Bartoš, M. - Foltán, R. - Mikšík, Ivan . Differences of Saliva Composition in Relation to Tooth Decay and Gender . Folia Biologica 2018, 64(5-6), 195-203 . IF = 1.073 [ASEP]
Kohutiar, Matej - Eckhardt, Adam - Mikšík, Ivan - Šantorová, J. - Wilhelm, Jiří . Proteomic analysis of peroxynitrite-induced protein nitration in isolated beef heart mitochondria . Physiological Research 2018, roč. 67, 2, p. 239-250 . IF = 1.701 [ASEP]
Ergang, Peter - Mikulecká, Anna - Vodička, Martin - Vagnerová, Karla - Mikšík, Ivan - Pácha, Jiří . Social defeat stimulates local glucocorticoid regeneration in lymphoid organs . Endocrine Connections 2018, 7(12), 1389-1396 . IF = 2.474 [ASEP] [ doi ]
Eckhardt, Adam - Kulhavá, Lucie - Mikšík, Ivan - Pataridis, Statis - Hlaváčková, Markéta - Vašinová, Jana - Kolář, František - Sedmera, David - Ošťádal, Bohuslav . Proteomic analysis of cardiac ventricles: baso-apical differences . Molecular and Cellular Biochemistry 2018, roč. 445, 1-2, p. 211-219 . IF = 2.884 [ASEP] [ doi ]
Svobodová, Jana - Kofroňová, Olga - Benada, Oldřich - Král, V. - Mikšík, Ivan . Separation of oligopeptides, nucleobases, nucleosides and nucleotides using capillary electrophoresis/electrochromatography with sol–gel modified inner capillary wall . Journal of Chromatography A. 2017, roč. 1517, p. 185-194 . IF = 3.716 [ASEP] [ doi ]
Ošťádal, M. - Lišková, Jana - Hadraba, Daniel - Eckhardt, Adam . Possible Pathogenetic Mechanisms and New Therapeutic Approaches of Pes Equinovarus . Physiological Research. 2017, roč. 66, 3, p. 403-410 . IF = 1.324 [ASEP]
Mikšík, Ivan . Capillary electrochromatography of proteins and peptides (2006–2015) . Journal of Separation Science. 2017, roč. 40, 1, p. 251-271 . IF = 2.415 [ASEP] [ doi ]
Mikšík, Ivan - Sedláková, Pavla - Pataridis, Statis - Bortolotti, F. - Gottardo, R. Proteins and their modifications in a medieval mummy . Protein Science. 2016, roč. 25, 11, p. 2037-2044 . IF = 2.523 [ASEP] [ doi ]
Jágr, Michal - Eckhardt, Adam - Pataridis, Statis - Foltán, R. - Myšák, J. - Mikšík, Ivan . Proteomic analysis of human tooth pulp proteomes – Comparison of caries-resistant and caries-susceptible . Journal of Proteomics. 2016, roč. 145, Aug 11, p. 127-136 . IF = 3.914 [ASEP] [ doi ]
Duval, C. - Cassey, P. - Lovell, P.G. - Mikšík, Ivan - Reynolds, S.J. - Spencer, K.A. Maternal influence on eggshell maculation: implications for cryptic camouflaged eggs . Journal of Ornithology. 2016, roč. 157, 1, p. 303-310 . IF = 1.468 [ASEP] [ doi ]
Ošťádal, M. - Eckhardt, Adam - Herget, J. - Mikšík, Ivan - Dungl, P. - Chomiak, J. - Frydrychová, M. - Burian, M. Proteomic analysis of the extracellular matrix in idiopathic pes equinovarus . Molecular and Cellular Biochemistry. 2015, Vol. 401, 1-2, p. 133-139 . IF = 2.613 [ASEP] [ doi ]
Maurer, G. - Portugal, S. J. - Hauber, M. E. - Mikšík, Ivan - Russell, D. G. D. - Cassey, P. First light for avian embryos: eggshell thickness and pigmentation mediate variation in development and UV exposure in wild bird eggs . Functional Ecology. 2015, Vol. 29, 2, p. 209-218 . IF = 5.21 [ASEP] [ doi ]
Ujčíková, Hana - Eckhardt, Adam - Kagan, Dmytro - Roubalová, Lenka - Svoboda, Petr . Proteomic analysis of post-nuclear supernatant fraction and percoll-purified membranes prepared from brain cortex of rats exposed to increasing doses of morphine . Proteome Science 2014, roč. 12, feb 14, p. 11. ISSN 1477-5956. IF = 1.725 [ASEP] [ doi ]
Mikšík, Ivan - Sedláková, Pavla - Pataridis, Statis - Bortolotti, F. - Gottardo, R. - Tagliaro, F. Prince Cangrande’s Collagen: Study of Protein Modification on the Mummy of the Lord of Verona, Italy (1291–1329 AD) . Chromatographia 2014, roč. 77, 21-22, p. 1503-1510. ISSN 0009-5893. IF = 1.411 [ASEP] [ doi ]
Mikšík, Ivan - Ergang, Peter - Pácha, Jiří . Proteomic analysis of chicken eggshell cuticle membrane layer . Analytical and Bioanalytical Chemistry 2014, roč. 406, 29, p. 7633-7640. ISSN 1618-2642. IF = 3.436 [ASEP] [ doi ]
Jágr, Michal - Eckhardt, Adam - Pataridis, Statis - Broukal, Z. - Dušková, J. - Mikšík, Ivan . Proteomics of human teeth and saliva . Physiological Research 2014, roč. 63, suppl.1, s141-s154. ISSN 0862-8408. IF = 1.293 [ASEP]
Houštěk, Josef - Vrbacký, Marek - Hejzlarová, Kateřina - Zídek, Václav - Landa, Vladimír - Šilhavý, Jan - Šimáková, Miroslava - Mlejnek, Petr - Kazdová, L. - Mikšík, Ivan - Neckář, Jan - Papoušek, František - Kolář, František - Kurtz, T. W. - Pravenec, Michal . Effects of mtDNA in SHR-mtF344 versus SHR conplastic strains on reduced OXPHOS enzyme levels, insulin resistance, cardiac hypertrophy, and systolic dysfunction . Physiological Genomics 2014, roč. 46, 18, p. 671-678. ISSN 1094-8341. IF = 2.374 [ASEP] [ doi ]
Hlaváček, Jan - Vítovcová, M. - Sázelová, Petra - Pícha, Jan - Vaněk, Václav - Buděšínský, Miloš - Jiráček, Jiří - Gillner, D. M. - Holz, R. C. - Mikšík, Ivan - Kašička, Václav . Mono-N-acyl-2,6-diaminopimelic acid derivatives: Analysis by electromigration and spectroscopic methods and examination of enzyme inhibitory activity . Analytical Biochemistry 2014, roč. 467, dec 15, p. 4-13. ISSN 0003-2697. IF = 2.219 [ASEP] [ doi ]
Fargallo, J. A. - López-Rull, I. - Mikšík, Ivan - Eckhardt, Adam - Peralta-Sánchez, J. M. Eggshell pigmentation has no evident effects on offspring viability in common kestrels . Evolutionary Ecology 2014, roč. 28, 4, p. 627-637. ISSN 0269-7653. IF = 2.517 [ASEP] [ doi ]
Eckhardt, Adam - Jágr, Michal - Pataridis, Statis - Mikšík, Ivan . Proteomic Analysis of Human Tooth Pulp: Proteomics of Human Tooth . Journal of Endodontics. 2014, roč. 40, 12, p. 1961-1966. ISSN 0099-2399 . IF = 3.375 [ASEP] [ doi ]
Duval, C. - Zimmer, C. - Mikšík, Ivan - Cassey, P. - Spencer, K.A. Early life stress shapes female reproductive strategy through eggshell pigmentation in Japanese quail . General and Comparative Endocrinology 2014, roč. 208, -, p. 146-153. ISSN 0016-6480. IF = 2.47 [ASEP] [ doi ]
Brulez, K. - Cassey, P. - Meeson, A. - Mikšík, Ivan - Webber, S.L. - Gosler, A.G. - Reynolds, S.J. Eggshell spot scoring methods cannot be used as a reliable proxy to determine pigment quantity . Journal of avian biology 2014, roč. 45, 1, p. 94-102. ISSN 0908-8857. IF = 1.971 [ASEP] [ doi ]
Pataridis, Statis - Šťastná, Zdeňka - Sedláková, Pavla - Mikšík, Ivan . Monotopic modifications derived from in vitro glycation of albumin with ribose . Electrophoresis, 2013, Vol. 34, 12, p. 1757-1763 . IF = 3.261 [ASEP] [ doi ]
Duval, C. - Cassey, P. - Lovell, P.G. - Mikšík, Ivan - Reynolds, S.J. - Spencer, K.A. Eggshell Appearance Does Not Signal Maternal Corticosterone Exposure in Japanese Quail: An Experimental Study with Brown-Spotted Eggs . PLoS ONE, 2013, Vol. 8, 12, e80485 . IF = 3.730 [ASEP] [ doi ]
Duval, C. - Cassey, P. - Mikšík, Ivan - Reynolds, S.J. - Spencer, K.A. Condition-dependent strategies of eggshell pigmentation: an experimental study of Japanese quail (Coturnix coturnix japonica) . Journal of Experimental Biology, 2013, Vol. 216, 4, p. 700-708 . IF = 3.236 [ASEP] [ doi ]
Brabcová, Jana - Kindl, Jiří - Valterová, Irena - Pichová, Iva - Zarevúcka, Marie - Brabcová, J. - Jágr, Michal - Mikšík, Ivan . Serine protease from midgut of Bombus terrestris males . Archives of Insect Biochemistry and Physiology, 2013, Vol. 82, 3, p. 117-128 . IF = 1.515 [ASEP] [ doi ]
Brabcová, Jana - Demianova, Zuzana - Vondrášek, Jiří - Jágr, Michal - Zarevúcka, Marie - Palomo, J. M. Highly selective purification of three lipases from Geotrichum candidum 4013 and their characterization and biotechnological applications . Journal of Molecular Catalysis B-Enzymatic, 2013, Vol. 98, Dec 30, p. 62-72 . IF = 2.823 [ASEP] [ doi ]
Řezanka, P. - Ehala, Sille - Koktan, J. - Sýkora, D. - Žvátora, P. - Vosmanská, M. - Král, V. - Mikšík, Ivan - Čeřovský, Václav - Kašička, Václav . Application of Bare Gold Nanoparticles in Open-Tubular CEC Separations of Polyaromatic Hydrocarbons and Peptides . Journal of Separation Science, 2012, Vol. 35, 1, p. 73-78 . IF = 2.591 [ASEP] [ doi ]
Neckář, Jan - Boudíková, Adéla - Mandíková, Petra - Štěrba, M. - Popelová, O. - Mikšík, Ivan - Dabrowská, L. - Mráz, J. - Geršl, V. - Kolář, František . Protective effects of dexrazoxane against acute ischaemia/reperfusion injury of rat hearts . Canadian Journal of Physiology and Pharmacology, 2012, Vol. 90, 9, p. 1303-1310 . IF = 1.556 [ASEP] [ doi ]
Mikšík, Ivan - Lacinová, Kateřina - Zmatlíková, Zdeňka - Sedláková, Pavla - Král, V. - Sýkora, D. - Řezanka, P. - Kašička, Václav . Open-tubular capillary electrochromatography with bare gold nanoparticles-based stationary phase applied to separation of trypsin digested native and glycated proteins . Journal of Separation Science, 2012, Vol. 35, 8, p. 994-1002 . IF = 2.591 [ASEP] [ doi ]
Mikulíková, Kateřina - Romanov, O. - Mikšík, Ivan - Eckhardt, Adam - Pataridis, Statis - Sedláková, Pavla . Study of Saiga Horn Using High-Performance Liquid Chromatography with Mass Spectrometry . Scientific World Journal, 2012, Vol. 2012, 2012, ID759604 . IF = 1.730 [ASEP] [ doi ]
Liotta, E. - Gottardo, R. - Seri, C. - Rimondo, C. - Mikšík, Ivan - Serpelloni, G. - Tagliaro, F. Rapid analysis of caffeine in “smart drugs” and “energy drinks” by microemulsion electrokinetic chromatography (MEEKC) . Forensic Science International, 2012, Vol. 220, 1-3, p. 279-283 . IF = 2.307 [ASEP] [ doi ]
Jágr, Michal - Eckhardt, Adam - Pataridis, Statis - Mikšík, Ivan . Comprehensive proteomic analysis of human dentin . European Journal of Oral Sciences, 2012, Vol. 120, 4, p. 259-268 . IF = 1.420 [ASEP] [ doi ]
Houštěk, Josef - Hejzlarová, Kateřina - Vrbacký, Marek - Drahota, Zdeněk - Landa, Vladimír - Zídek, Václav - Mlejnek, Petr - Šimáková, Miroslava - Šilhavý, Jan - Mikšík, Ivan - Kazdová, L. - Oliyarnyk, O. - Kurtz, T. - Pravenec, Michal . Nonsynonymous variants in mt-Nd2, mt-Nd4, and mt-Nd5 are linked to effects on oxidative phosphorylation and insulin sensitivity in rat conplastic strains . Physiological Genomics, 2012, Vol. 44, 9, p. 487-494 . IF = 2.806 [ASEP] [ doi ]
Gottardo, R. - Mikšík, Ivan - Aturki, Z. - Sorio, D. - Seri, C. - Fanali, S. - Tagliaro, F. Analysis of drugs of forensic interest with capillary zone electrophoresis/time-of-flight mass spectrometry based on the use of non-volatile buffers . Electrophoresis, 2012, Vol. 33, 4, p. 599-606 . IF = 3.261 [ASEP] [ doi ]
Cassey, P. - Thomas, G.H. - Portugal, S. J. - Maurer, G. - Hauber, M. E. - Grim, T. - Lovell, P.G. - Mikšík, Ivan . Why are birds' eggs colourful? Eggshell pigments co-vary with life-history and nesting ecology among British breeding non-passerine birds . Biological Journal of the Linnean Society, 2012, Vol. 106, 3, p. 657-672 . IF = 2.413 [ASEP] [ doi ]
Cassey, P. - Mikšík, Ivan - Portugal, S. J. - Maurer, G. - Ewen, J.G. - Zarate, E. - Sewell, M.A. - Karadas, F. - Grim, T. - Hauber, M. E. Avian eggshell pigments are not consistently correlated with colour measurements or egg constituents in two Turdus thrushes . Journal of avian biology, 2012, Vol. 43, 6, p. 503-512 . IF = 2.020 [ASEP] [ doi ]
Řezanka, P. - Navrátilová, K. - Žvátora, P. - Sýkora, D. - Matějka, P. - Mikšík, Ivan - Kašička, Václav - Král, V. Cyclodextrin modified gold nanoparticles-based open-tubular capillary electrochromatographic separations of polyaromatic hydrocarbons . Journal of Nanoparticle Research, 2011, Vol. 13, 11, p. 5947-5957 . IF = 3.287 [ASEP] [ doi ]
Vagnerová, Karla - Loukotová, Jana - Ergang, Peter - Musílková, Jana - Mikšík, Ivan - Pácha, Jiří . Peroxisome proliferator-activated receptor-gamma stimulates 11beta-hydroxysteroid dehydrogenase type 1 in rat vascular smooth muscle cells . Steroids, 2011, Vol. 76, 6, p. 577-581 . IF = 2.829 [ASEP] [ doi ]
Maurer, G. - Portugal, S. J. - Mikšík, Ivan - Cassey, P. Speckles of cryptic black-headed gull eggs show no mechanical or conductance structural function . Journal of Zoology, 2011, Vol. 285, 3, p. 194-204 . IF = 2.043 [ASEP] [ doi ]
Klusoňová, Petra - Pátková, Lenka - Ergang, Peter - Mikšík, Ivan - Zicha, Josef - Kuneš, Jaroslav - Pácha, Jiří . Local metabolism of glucocorticoids in Prague hereditary hypertriglyceridemic rats – Effect of hypertriglyceridemia and gender . Steroids, 2011, Vol. 76, 12, p. 1252-1259 . IF = 2.829 [ASEP] [ doi ]
Grausová, Ľubica - Kromka, Alexander - Burdíková, Zuzana - Eckhardt, Adam - Rezek, Bohuslav - Vacík, Jiří - Haenen, K. - Lisá, Věra - Bačáková, Lucie . Enhanced Growth and Osteogenic Differentiation of Human Osteoblast-Like Cells on Boron-Doped Nanocrystalline Diamond Thin Films . PLoS ONE, 2011, Vol. 6, 6, e20943 . IF = 4.092 [ASEP] [ doi ]
Ergang, Peter - Vytáčková, Kateřina - Švec, Jiří - Bryndová, Jana - Mikšík, Ivan - Pácha, Jiří . Upregulation of 11beta-hydroxysteroid dehydrogenase 1 in lymphoid organs during inflammation in the rat . Journal of Steroid Biochemistry and Molecular Biology, 2011, Vol. 126, 1-2, p. 19-25 . IF = 3.053 [ASEP] [ doi ]
Zmatlíková, Zdeňka - Sedláková, Pavla - Lacinová, Kateřina - Eckhardt, Adam - Pataridis, Statis - Mikšík, Ivan . Non-enzymatic posttranslational modifications of bovine serum albumin by oxo-compounds investigated by high-performance liquid chromatography–mass spectrometry and capillary zone electrophoresis–mass spektrometry . Journal of Chromatography A, 2010, Vol. 1217, 51, p. 8009-8015 . IF = 4.194 [ASEP] [ doi ]
Sýkora, D. - Kašička, Václav - Mikšík, Ivan - Řezanka, P. - Záruba, K. - Matějka, P. - Král, V. Application of gold nanoparticles in separation sciences . Journal of Separation Science, 2010, Vol. 33, 3, p. 372-387 . IF = 2.631 [ASEP] [ doi ]
Mikšík, Ivan - Sedláková, Pavla - Lacinová, Kateřina - Pataridis, Statis - Eckhardt, Adam . Determination of insoluble avian eggshell matrix proteins . Analytical and Bioanalytical Chemistry, 2010, Vol. 397, 1, p. 205-214 . IF = 3.841 [ASEP] [ doi ]
Maxová, H. - Bačáková, Lucie - Eckhardt, Adam - Mikšík, Ivan - Lisá, Věra - Novotná, J. - Herget, J. Growth of Vascular Smooth Muscle Cells on Collagen I Exposed to RBL-2H3 Mastocytoma Cells . Cellular Physiology and Biochemistry, 2010, Vol. 25, 6, p. 615-622 . IF = 3.585 [ASEP] [ doi ]
Klásek, A. - Lyčka, A. - Mikšík, Ivan - Růžička, A. Reaction of 3-phenyl-3-aminoquinoline-2,4-diones with isothiocyanates. Facile access to novel spiro-linked 2-thioxoimidazolidine-oxindoles and imidazoline-2-thiones . Tetrahedron, 2010, Vol. 66, 11, p. 2015-2025 . IF = 3.011 [ASEP] [ doi ]
Jeleník, Tomáš - Rossmeisl, Martin - Kuda, Ondřej - Macek Jílková, Zuzana - Medříková, Daša - Kůs, Vladimír - Hensler, Michal - Janovská, Petra - Mikšík, Ivan - Baranowski, M. - Gorski, J. - Hébrard, S. - Jensen, T. E. - Flachs, Pavel - Hawley, S. - Violl . AMP-activated Protein Kinase alfa2 Subunit Is Required for the Preservation of Hepatic Insulin Sensitivity by n-3 Polyunsaturated Fatty Acids . Diabetes, 2010, Vol. 59, 11, p. 2737-2746 . IF = 8.889 [ASEP] [ doi ]
Furuhashi, T. - Mikšík, Ivan - Smrž, Miloslav - Germann, B. - Nebija, D. - Lachmann, B. - Noe, Ch. Comparison of aragonitic molluscan shell proteins . Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology, 2010, Vol. 155, 2, p. 195-200 . IF = 1.989 [ASEP] [ doi ]
Ergang, Peter - Leden, P. - Vagnerová, Karla - Klusoňová, Petra - Mikšík, Ivan - Jurčovičová, J. - Kment, M. - Pácha, Jiří . Local metabolism of glucocorticoids and its role in rat adjuvant arthritis . Molecular and Cellular Endocrinology, 2010, Vol. 323, 2, p. 155-160 . IF = 4.119 [ASEP] [ doi ]
Vacková, Z. - Vagnerová, Karla - Libra, A. - Mikšík, Ivan - Pácha, Jiří - Staud, F. Dexamethasone and betamethasone administration during pregnancy affects expression and function of 11beta-hydroxysteroid dehydrogenase type 2 in the rat placenta . Reproductive Toxicology, 2009, Vol. 28, 1, p. 46-51 . IF = 3.367 [ASEP] [ doi ]
Sedláková, Pavla - Eckhardt, Adam - Lacinová, Kateřina - Pataridis, Statis - Mikšík, Ivan - Král, V. - Kašička, Václav . Separation of tryptic peptides of native and glycated BSA using open-tubular CEC with salophene-lanthanide-Zn2+ complex as stationary phase . Journal of Separation Science, 2009, Vol. 32, 22, p. 3930-3935 . IF = 2.551 [ASEP] [ doi ]
Prucková, Z. - Klásek, A. - Lyčka, A. - Mikšík, Ivan - Růžička, A. Synthesis of 2-thioxoimidazolines via reaction of 1-unsubstituted 3-aminoquinoline-2,4-diones with isothiocyanates . Tetrahedron, 2009, Vol. 65, 45, p. 9103-9115 . IF = 3.219 [ASEP] [ doi ]
Pataridis, Statis - Eckhardt, Adam - Mikulíková, Kateřina - Sedláková, Pavla - Mikšík, Ivan . Determination and Quantification of Collagen Types in Tissues Using HPLC-MS/MS . Current Analytical Chemistry, 2009, Vol. 5, 4, p. 316-323 . IF = 2.143 [ASEP] [ doi ]
Klásek, A. - Mrkvička, V. - Lyčka, A. - Mikšík, Ivan - Růžička, A. Reaction of 1-substituted 3-aminoquinoline-2,4-diones with isothiocyanates. An easy pathway to generate novel 2-thioxo-1′H-spiro[imidazoline-5,3′-indole]-2,2′-diones . Tetrahedron, 2009, Vol. 65, 25, p. 4908-4916 . IF = 3.219 [ASEP] [ doi ]
Klásek, A. - Lyčka, A. - Mikšík, Ivan - Růžička, A. Molecular Rearrangement of 9b-Hydroxy-1H-imidazo[4,5-c]quinoline-2,4-diones - A Convenient Pathway to Spiro-Linked Imidazolidine-Oxindole Derivatives . Helvetica Chimica Acta, 2009, Vol. 92, 4, p. 689-708 . IF = 1.435 [ASEP] [ doi ]
Klusoňová, Petra - Řeháková, Lenka - Borchert, Gudrun H. - Vagnerová, Karla - Neckář, Jan - Ergang, Peter - Mikšík, Ivan - Kolář, František - Pácha, Jiří . Chronic Intermittent Hypoxia Induces 11Beta-Hydroxysteroid Dehydrogenase in Rat Heart . Endocrinology, 2009, Vol. 150, 9, p. 4270-4277 . IF = 4.752 [ASEP] [ doi ]
Furuhashi, T. - Schwarzinger, C. - Mikšík, Ivan - Smrž, Miloslav - Beran, A. Molluscan shell evolution with review of shell calcification hypothesis . Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology, 2009, Vol. 154, 3, p. 351-371 . IF = 1.607 [ASEP] [ doi ]
Vagnerová, Karla - Vacková, Z. - Klusoňová, Petra - Štaud, F. - Kopecký, M. - Ergang, Peter - Mikšík, Ivan - Pácha, Jiří . Reciprocal Changes in Maternal and Fetal Metabolism of Corticosterone in Rat During Gestation . Reproductive Sciences, 2008, Vol. 15, 9, p. 921-931 . IF = 1.951 [ASEP]
Roulin, A. - Almasi, B. - Rossi-Pedruzzi, A. - Ducrest, A.-L. - Wakamatsu, K. - Mikšík, Ivan - Blount, J.D. - Jenni-Eiermann, S. - Jenni, L. Corticosterone mediates the condition-dependent component of melanin-based coloration . Animal Behaviour, 2008, Vol. 75, 4, p. 1351-1358 . IF = 2.828 [ASEP]

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People

doc. Ing. Tomáš Čajka, Ph.D.

Head of the Laboratory

Mgr. Adam Eckhardt, Ph.D.

Deputy Head of the Laboratory

prof. Ing. Ivan Mikšík, DrSc.

Senior Researcher

Ing. Jana Svobodová, Ph.D.

Senior Researcher

Ing. Statis Pataridis

Research Assistant

Mgr. Lucie Rudl Kulhavá, Ph.D.

Postdoctoral Fellow

MSc. Marine Morvan

PhD Student

Mgr. Armenuhi Kirakosjanová

Technician