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Laboratory of Structural Biology of Signaling Proteins

Laboratory of Structural Biology of Signaling Proteins

Our Laboratory is focused on structural biology (the relationship between the structure and function of certain groups of proteins), particularly we focus on the proteins which participate in the signal transmission in the cell. Among methods we use are recombinant protein expression, biophysical characterization, study of intermolecular interactions, protein structure and interaction surfaces. All these methods enable us to better understand the details how the activity and function of protein-protein complexes is regulated. Our research is focused mainly on:

  • Structural biology of 14-3-3 proteins and their complexes
  • Study of inhibition of ubiquitin ligase Nedd4-2 by 14-3-3 protein
  • Characterizaton of the interactions between transcription factor FOXO4 and tumour supressor p53
  • Mechanism of regulation of proteinkinase ASK1 by TRX and 14-3-3 protein
  • Mechanism of regulation of protease caspase-2 by 14-3-3 protein






Transcription factor p53 protects cells against tumorigenesis when subjected to various cellular stresses. Under stress conditions, p53 interacts with another transcription factor, FOXO4 (Forkhead box O 4), and together they increase the production of p21 protein, which triggers the process of cell aging (senescence). However, the molecular mechanism of upregulation of p21 transcription is still unclear. In a work published in the Protein Science journal, scientific teams of Dr. Obsilova (IPHYS CAS), prof. Obsil (Faculty of Science, Charles University and IPHYS CAS) and their colleagues from IOCB CAS characterized interactions between p53 and FOXO4 at the molecular level. New knowledge about the structure of the complex may enable the development of specific inhibitors of the interaction between these two proteins, and subsequently in the development of new drugs aimed at the selective elimination of senescent cells.   More
Our new articles in Communications Biology

Our new articles in Communications Biology

Two accepted papers in Communications Biology: July and August 2021 Pavel Pohl, Rohit Joshi, Olivia Petrvalska, Tomas Obsil and Veronika Obsilova 14-3-3-protein regulates Nedd4-2 by modulating interactions between HECT and WW domains | Communications Biology ( Commun. Biol. 2021 July 22; 4(1):899. Matej Horvath, Olivia Petrvalska, Petr Herman, Tomas Obsil and Veronika Obsilova 14-3-3 proteins inactivate DAPK2 by promoting its dimerization and protecting key regulatory phosphosites | Communications Biology ( Commun. Biol. 2021 August 19; 4(1):986. IF = 6.268 More

Tomáš Obšil was awarded the title of professor

Prof. RNDr. Tomáš Obšil, Ph.D. was awarded by the title of professor at The Faculty of Science, Charles University, majoring in Physical Chemistry. Professors graduation will take place December 18, 2014. More


Obšilová; Veronika - Obšil; T. The yeast 14-3-3 proteins Bmh1 and Bmh2 regulate key signaling pathways . Frontiers in molecular biosciences. 2024; 11(Jan 24); 1327014 . IF = 5.0 [ASEP] [ doi ]
Petrvalská; Olivia - Honzejková; K. - Koupilová; N. - Herman; P. - Obšilová; Veronika - Obšil; Tomáš . 14-3-3 protein inhibits CaMKK1 by blocking the kinase active site with its last two C-terminal helices . Protein Science. 2023; 32(11); e4805 . IF = 8.0 [ASEP] [ doi ]
Masaryk; Jakub - Kale; Deepika - Pohl; Pavel - Ruiz-Castilla; F. J. - Zimmermannová; Olga - Obšilová; Veronika - Ramos; J. - Sychrová; Hana . The second intracellular loop of the yeast Trk1 potassium transporter is involved in regulation of activity; and interaction with 14–3-3 proteins . Computational and Structural Biotechnology Journal. 2023; 21(April); 2705-2716 . IF = 6.0 [ASEP] [ doi ]
Obšilová; Veronika - Obšil; T. Structural insights into the functional roles of 14-3-3 proteins . Frontiers in molecular biosciences. 2022; 9(Sep 16)); 1016071 . IF = 5.0 [ASEP] [ doi ]
Mandal; R. - Kohoutová; Klára - Petrvalská; Olivia - Horváth; M. - Srb; Pavel - Veverka; Václav - Obšilová; Veronika - Obšil; Tomáš . FOXO4 interacts with p53 TAD and CRD and inhibits its binding to DNA . Protein Science. 2022; 31(5)); e4287 . IF = 8.0 [ASEP] [ doi ]
Kohoutová; Klára - Dočekal; V. - Ausserlechner; M. J. - Kaiser; N. - Tekel; A. - Mandal; R. - Horváth; M. - Obšilová; Veronika - Veselý; J. - Hagenbuchner; J. - Obšil; Tomáš . Lengthening the Guanidine–Aryl Linker of Phenylpyrimidinylguanidines Increases Their Potency as Inhibitors of FOXO3-Induced Gene Transcription . ACS Omega. 2022; 7(38); 34632-34646 . IF = 4.2 [ASEP] [ doi ]
Joshi; Rohit - Pohl; Pavel - Strachotová; D. - Herman; P. - Obšil; Tomáš - Obšilová; Veronika . Nedd4-2 binding to 14-3-3 modulates the accessibility of its catalytic site and WW domains . Biophysical Journal. 2022; 121(7); 1299-1311 . IF = 3.4 [ASEP] [ doi ]
Pohl; Pavel - Joshi; Rohit - Petrvalská; Olivia - Obšil; Tomáš - Obšilová; Veronika . 14-3-3-protein regulates Nedd4-2 by modulating interactions between HECT and WW domains . Communications Biology. 2021; 4(1)); 899 . IF = 6.548 [ASEP] [ doi ]
Obšilová; Veronika - Honzejková; K. - Obšil; Tomáš . Structural Insights Support Targeting ASK1 Kinase for Therapeutic Interventions . International Journal of Molecular Sciences. 2021; 22(24)); 13395 . IF = 6.208 [ASEP] [ doi ]
Neves; J. F. - Petrvalská; Olivia - Bosica; F. - Cantrelle; F. X. - Merzougui; H. - O'Mahony; G. - Hanoulle; X. - Obšil; Tomáš - Landrieu; I. Phosphorylated full-length Tau interacts with 14-3-3 proteins via two short phosphorylated sequences; each occupying a binding groove of 14-3-3 dimer . FEBS Journal. 2021; 288(6); 1918-1934 . IF = 5.622 [ASEP] [ doi ]

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 RNDr. Veronika Obšilová, Ph.D.
 Head of the Laboratory
Prof. RNDr. Tomáš Obšil, Ph.D.
Deputy Head of the Laboratory (part time)

MgrDalibor Košek, Ph.D.
Senior Researcher
M.Sc. Maša Janošev
PhD Student
M.Sc. Jayashri Bhosale
PhD Student
M.Sc. Klára Kohoutová
ext. - PhD student

M.Sc. Andrej Tekel
ext. - PhD Student

Bc. Adam Brzezina
ext. - Undergraduate Student

Bc. Matúš Friček
ext.- Undergraduate Student
Bc. Martin Hýbl
ext. - Undergraduate Student
Mgr. Dana Kalábová, Ph.D.
Bc. Gabriela Kočárová