Cutting-edge science for health

Laboratory of Structural Biology of Signaling Proteins

Laboratory of Structural Biology of Signaling Proteins

Our Laboratory is focused on structural biology (the relationship between the structure and function of certain groups of proteins), particularly we focus on the proteins which participate in the signal transmission in the cell. Among methods we use are recombinant protein expression, biophysical characterization, study of intermolecular interactions, protein structure and interaction surfaces. All these methods enable us to better understand the details how the activity and function of protein-protein complexes is regulated. Our research is focused mainly on:

  • Structural biology of 14-3-3 proteins and their complexes
  • Mechanism of regulation of proteinkisases CaMKK1, CaMKK2 and ASK1
  • Study of inhibition of protease caspase-2 by 14-3-3 protein
  • Study of DNA-binding domain of transcription factor FOXO4





Valenti; D. - Neves; J. F. - Cantrelle; F. X. - Hristeva; S. - Santo Lentini; D. - Obšil; Tomáš - Hanoulle; X. - Levy; L. M. - Tzalis; D. - Landrieu; I. - Ottmann; Ch. Set-up and screening of a fragment library targeting the 14-3-3 protein interface . MedChemComm. 2019; 10(10); 1796-1802 . IF = 2.394 [ASEP] [ doi ]
Šmídová; Aneta - Staňková; Kateřina - Petrvalská; Olivia - Lazar; Josef - Sychrová; Hana - Obšil; Tomáš - Zimmermannová; Olga - Obšilová; Veronika . The activity of Saccharomyces cerevisiae Na+; K+/H+ antiporter Nha1 is negatively regulated by 14-3-3 protein binding at serine 481 . Biochimica Et Biophysica Acta-Molecular Cell Research. 2019; 1866(12)); 118534 . IF = 4.739 [ASEP] [ doi ]
Pšenáková; Katarína - Kohoutová; K. - Obšilová; Veronika - Ausserlechner; M. J. - Veverka; Václav - Obšil; Tomáš . Forkhead Domains of FOXO Transcription Factors Differ in both Overall Conformation and Dynamics . Cells. 2019; 8(9)); 966 . IF = 5.656 [ASEP] [ doi ]
He; F. - Mori; T. - Morita; I. - Nakamura; H. - Alblová; Miroslava - Hoshino; S. - Awakawa; T. - Abe; I. Molecular basis for the P450-catalyzed C-N bond formation in indolactam biosynthesis . Nature Chemical Biology. 2019; 15(12); 1206-1213 . IF = 12.154 [ASEP] [ doi ]
Hagenbuchner; J. - Obšilová; Veronika - Kaserer; T. - Kaiser; N. - Rass; B. - Pšenáková; Katarína - Dočekal; V. - Alblová; Miroslava - Kohoutová; Klára - Schuster; D. - Aneichyk; T. - Veselý; J. - Obexer; P. - Obšil; Tomáš - Ausserlechner; M. J. Modulating FOXO3 transcriptional activity by small; DBD-binding molecules . eLife. 2019; 8(Dec 4)); e48876 . IF = 7.551 [ASEP] [ doi ]

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 RNDr. Veronika Obšilová, Ph.D.
 Head of the Laboratory
Prof. RNDr. Tomáš Obšil, Ph.D.
Deputy Head of the Laboratory (part time)

RNDrOlívia Petrvalská, Ph.D.
Junior Researcher
Mgr. Matěj Horváth, Ph.D.
Junior Researcher (part time)
Mgr. Dana Kalábová (born Žáková) 
PhD Student
Mgr. Pavel Pohl
PhD Student
M.Sc. Rohit Ashok Joshi
PhD Student
MSc. Raju Mandal
PhD Student (part time)
M.Sc. Karolína Honzejková
PhD Student (part time)
Bc. Klára Kohoutová
Undergraduate Student
Bc. Nicola Koupilová
Undergraduate Student
Bc. Martina Mikulů
Undergraduate Student
Adéla Hofmanová
Undergraduate Student

Andrej Tekel

Undergraduate Student

Markéta Kolcunová